摘要
该研究对2020年浙江设施栽培的番茄进行病害调查,利用柯赫氏法则进行病原菌的致病性测定,采用形态观察、革兰氏染色法、脂肪酸含量测定及16Sr RNA-gyr B-rpoB-rpo D四基因联合系统发育分析等方法对分离的病原菌进行种属鉴定,并进行药剂室内毒力测定。该研究表明番茄髓部坏死病有明显的发病中心,设施大棚中易传播扩散;经形态学、生理生化和分子鉴定等方法确认该病原菌为绿黄假单胞菌(Pseudomonas viridiflava);中生菌素对番茄髓部坏死病菌的抑制效果较好,其EC50仅0.106 mg/L,是较为理想的杀菌剂。
2020,the tomato disease field investigation in greenhouses of Zhejiang province is conducted.Koch's rule is used to determine the pathogenicity of pathogens.Morphological observation,Gram staining,fatty acid profiling and 16S rRNA-gyrB-rpoB-rpoD four genes combined phylogenetic analysis are used to identify the species of isolated pathogens.The toxicity of the reagent is measured in laboratory.There are obvious infection centers of tomato pith necrosis,and it is easy to spread in the greenhouse.The pathogen is clearly supported as Pseudomonas viridiflava by morphological,physiological,biochemical and molecular identification.As EC50 of the inhibitory effect of Zhongshengmycin is only 0.106 mg/L,it would be an ideal fungicide for tomato pith necrosis.
作者
冯晓晓
施斌
陈恩磊
王国荣
吴慧明
郑永利
FENG Xiaoxiao;SHI Bin;CHEN Enlei;WANG Guorong;WU Huiming;ZHENG Yongli(Agricultural Experiment Station,Zhejiang University,Hangzhou 310058,China;College of Advanced Agriculture Sciences,Zhejiang Agricultural and Forestry University,Hangzhou 311300,China;Agro-forestry Technology Extension Center,Xiaoshan District,Hangzhou 311203,China;Zhejiang Agricultural Products Green Development Center,Hangzhou 310003,China)
出处
《实验技术与管理》
CAS
北大核心
2023年第3期14-20,共7页
Experimental Technology and Management
基金
浙江大学实验技术研究项目(SJS202019)
浙江省重点研发计划(2020C02027)
浙江省教育厅一般科研项目(Y202249712)
浙江省“三农九方”科技协作项目(2022SNJF015)。
关键词
番茄髓部坏死病
假单胞菌
病原鉴定
中生菌素
tomato pith necrosis
Pseudomonas
pathogen identification
Zhongshengmycin
