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马钱苷对严重烧伤大鼠肠黏膜损伤的保护作用及对TLR4/NF-κB通路的影响

Protective effect of loganin on intestinal mucosal injury and TLR4/NF-κB pathway in severely burned rats
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摘要 目的:探讨马钱苷对严重烧伤大鼠肠黏膜损伤的保护作用及对Toll样受体4(toll-like receptor 4,TLR4)/核转录因子-κB(nuclear transcription factor-κB,NF-κB)炎症通路的影响。方法:30只雄性SD大鼠随机分为对照组、烧伤组与马钱苷组,每组10只。将烧伤组和马钱苷组大鼠背部造成30%体表总面积(total body surface area,TBSA)III度烫伤,将对照组大鼠模拟致假烧伤。马钱苷组大鼠予以马钱苷(5 mg马钱苷溶于1 mL生理盐水中)50 mg/kg灌胃,1次/d,持续7 d;其余2组予以等量生理盐水灌胃,1次/d,持续7 d。给药结束后,采用紫外分光光度计酶联免疫吸附法检测各组大鼠血清二胺氧化酶(diamine oxidase,DAO)、乳酸脱氢酶(lactate dehydrogenase,LDH)含量;采用酶联免疫吸附法检测血清肿瘤坏死因子α(tumor necrosis factorα,TNF-α)、白细胞介素(interleukin,IL)-1β、IL-6的水平;采用苏木精-伊红染色对大鼠回肠组织形态进行观察;采用蛋白质印迹法及免疫组织化学法检测肠黏膜组织TLR4及NF-κB-p65表达。结果:对照组大鼠回肠黏膜组织未见充血、水肿等变化;烧伤组回肠黏膜受损明显,可见区域充血、水肿,伴有明显炎性细胞浸润;马钱苷组也存在水肿、充血等表现,但相比烧伤组较轻,炎症细胞浸润减少。烧伤组和马钱苷组血清DAO、LDH含量及TNF-α、IL-6、IL-1β水平均显著高于对照组(均P<0.05),马钱苷组血清DAO、LDH含量及TNF-α、IL-6、IL-1β水平显著低于烧伤组(均P<0.05)。烧伤组和马钱苷组大鼠肠黏膜组织TLR4、NF-κB-p65表达水平均显著高于对照组(均P<0.05),马钱苷组大鼠肠黏膜组织TLR4、NF-κB-p65表达水平均显著低于烧伤组(均P<0.05)。结论:马钱苷可能通过抑制TLR4/NF-κB信号通路而减轻烧伤大鼠炎症反应,并对肠黏膜损伤发挥保护作用。 Objective:To investigate the protective effect of loganin on intestinal mucosal injury in severely burned rats and the effect of Toll-like receptor 4(TLR4)/nuclear transcription factor-κB(NF-κB)inflammatory pathway.Methods:Thirty male SD rats were randomly divided into a control group,a burn group,and a loganin group,each group with 10 rats.The burn group and loganin group were subjected to 30%total body surface area(TBSA)III degree burn on the back,and the control group was subjected to simulated pseudoburn.The rats in the loganin group were intragastrically administered with 50 mg/kg loganin(5 mg loganin dissolved in 1 mL normal saline)once daily for 7 days.The other 2 groups were given the same amount of normal saline by gavage(once daily for 7 days).At the end of administration,the contents of diamine oxidase(DAO)and lactate dehydrogenase(LDH)in serum of rats in each group were detected by ultraviolet spectrophotometer enzyme-linked immunosorbent assay.The levels of tumor necrosis factor-α(TNF-α),interleukin(IL)-1β,and IL-6 in serum were detected by enzyme-linked immunosorbent assay.The morphology of ileum tissues were observed by hematoxylin-eosin staining.The protein contents of TLR4 and NF-κB-p65 in intestinal mucosa were detected by protein imprinting method and immunohistochemistry.Results:In the control group,there was no changes in ileum tissue of rats such as congestion and edema;in the burn group,the ileum tissue was significantly damaged,showing regional congestion,edema and obvious inflammatory cell infiltration.The loganin group also had edema and congestion,but compared with the burn group,the inflammatory cell infiltration was reduced.Serum DAO,LDH and TNF-α,IL-6,IL-1βlevels in the burn group and the loganin group were significantly higher than those in the control group(all P<0.05).Serum DAO,LDH and TNF-α,IL-6,IL-1βlevels in the loganin group were significantly lower than those in the burn group(all P<0.05).The expression levels of TLR4 and NF-κB-p65 protein in intestinal mucosa in the burn group and the loganin group was significantly higher than that in the control group(all P<0.05).The expression levels of TLR4 and NF-κB-p65 protein in intestinal mucosa in the loganin group was significantly lower than that in the burn group(all P<0.05).Conclusion:Loganin may reduce the inflammatory response of burn rats by inhibiting TLR4/NF-κB signaling pathway,and play a protective role in intestinal mucosal injury.
作者 温海玲 杨景哲 孟祥熙 张祥云 WEN Hailing;YANG Jingzhe;MENG Xiangxi;ZHANG Xiangyun(Department of Burn and Plastic Surgery,South District Hospital,Affiliated Hospital of Chengde Medical University,Chengde Hebei 067000;Department of Outpatient,Chengde Retired Cadres Rest Center of Hebei Military Region,Chengde Hebei 067000,China)
出处 《临床与病理杂志》 CAS 2023年第2期221-228,共8页 Journal of Clinical and Pathological Research
基金 承德市科学技术研究与发展计划项目(202006A165)。
关键词 马钱苷 烧伤 肠黏膜 炎症反应 Toll样受体4/核转录因子-κB信号通路 loganin burn intestinal mucosa inflammatory reaction Toll-like receptor 4/nuclear transcription factor-κB signaling pathway
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