SD大鼠骨髓基质干细胞的全骨髓培养与鉴定

Whole bone marrow culture and identification of bone marrow stromal stem cells in SD rats

目的:采用体外全骨髓培养法培养SD大鼠骨髓基质干细胞(BMSCs)并进行鉴定。方法:采用全骨髓贴壁法分离扩增大鼠骨髓基质干细胞,采用流式细胞仪检测细胞表面分子CD29、CD34、CD45的表达。结果:全骨髓培养法能迅速、有效地培养和获得大量骨髓基质干细胞,流式细胞仪检测显示CD29阳性率为99.0%,CD34阳性率1.5%,CD45阳性率0.8%。结论:全骨髓贴壁培养法是获得骨髓基质干细简捷、高效、经济的途径,可以获得较纯的BMSCs。

Objective:Bone marrow stromal stem cells(BMSCs)of SD rats were cultured and identified by the method of whole bone marrow culture in vitro.Methods:Mouse bone marrow stromal stem cells were isolated and enlarged by the whole bone marrow adherent method,and the expressions of CD29,CD34 and CD45 on the cell surface were detected by flow cytometry.Results:The whole bone marrow culture method can rapidly and effectively culture and obtain a large number of bone marrow stromal stem cells.The positive rate of CD29,CD34 and CD45 were 99.0%,1.5%and 0.8%respectively.Conclusion:The whole bone marrow adherent culture is a simple,efficient and economical way to obtain bone marrow stromal stem cells,and can obtain relatively pure BMSCs.