基于高通量测序的四种落叶果树根际土壤真核生物结构多样性分析

Diversity Analysis of Eukaryotic Structure in Rhizosphere Soil of Four Deciduous Fruit Trees Based on High-throughput Sequencing

为探究4种落叶果树土壤根际真核生物结构多样性,对石榴、桃树、银杏、樱桃土壤根际真核生物结构的多样性进行测序、分析。共得到2 368个OTUs,银杏样品的辛普森指数相对较低,香农指数相对较高,显示该样品群落多样性相对较高,银杏样品的ACE指数和Chaol指数都比其他样品高,表明银杏样品群落丰富度相对较高。在属水平上,石榴样品真菌群落有4个真菌优势菌属,主要是毛壳菌属(18.68%)和镰刀菌属(6.77%)。桃树样品真菌群落有5个真菌优势菌属,主要是镰刀菌属(8.87%)和毛壳菌属(7.00%)。樱桃样品真菌群落有6个真菌优势菌属,主要是毛壳菌属(22.66%)、镰刀菌属(15.91%)及被孢霉属(11.16%)。银杏样品真菌群落有7个真菌优势菌属,主要是毛壳菌属(9.08%)和Parastagonospora(5.83%)。4种果树样品的优势类群主要是真菌,不同果树样品根际土壤真菌群落差异较大。

The present paper aimed to investigate the diversity of eukaryotic structure in rhizosphere soil of 4 deciduous fruit trees.The diversity of eukaryotic structure in the rhizosphere soil of pomegranate,peach,ginkgo and cherry was sequenced.The results showed that 2368 OTUs were obtained by sequencing.The Shannon index of ginkgo was higher and the Simpson index was lower,which indicated that the eukaryotic structure diversity of ginkgo was high.The Chaol,ACE index of ginkgo were higher than others,which indicated that the richness of eukaryotic community of ginkgo was higher.There were 4 dominant fungal genera in the fungal community of pomegranate at the genus level,which mainly included 18.68%of Chaetomium,6.77%of Fusarium.There were 5 dominant fungal genera in the fungal community of peach at the genus level,which mainly included Fusarium(8.87%),Chaetomium(7.00%).There were 6 dominant fungal genera in the fungal community of cherry at the genus level,which mainly included Chaetomium(22.66%),Fusarium(15.91%),Mortierella(11.16%).There were 7 dominant fungal genera in the fungal community of ginkgo at the genus level,which mainly included Chaetomium(9.08%)、Parastagonospora(5.83%).The dominant groups of the four fruit samples were fungi,and the fungal communities in rhizosphere soil of different fruit samples were different.