黄皮新肉桂核酰胺B对C57BL/6小鼠的降血脂作用

Hypolipidemic Effect of Lansiumamide B Obtained from Wampee Seeds in C57BL/6 Mice

从黄皮核中提取分离,得到单体黄皮新肉桂酰胺B(Lansiumamide B,LB),并进行结构鉴定,通过细胞和小鼠实验验证,LB具有较强的体内外降脂效果。结果表明:LB能高效抑制α-淀粉酶、α-葡萄糖苷酶和胰脂肪酶的活性,IC 50分别为18.43、15.27和0.16 mg/mL;作用于油酸(OA)诱导的高脂HepG2细胞,浓度为5μg/mL时,甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、丙二醛(MDA)分别降低了30.77%、30.00%、58.33%、39.97%、38.84%和43.90%,高密度脂蛋白胆固醇(HDL-C)、过氧化氢酶(CAT)以及低密度脂蛋白受体(LDLR)分别提高了83.33%、253.37%、60.97%;LB饲喂于高脂小鼠6周后,体重都有明显的降低,与高脂组有明显差异(P<0.05);血清中的血脂四项,给药组剂量为60 mg/kg时,TC、TG和LDL-C分别降低了29.18%、23.69%、70.27%,HDL-C升高了50.51%,说明高脂小鼠的体重和血脂四项指标都得到了很好的控制。该研究初步表明LB具有较好的细胞和活体降血脂作用,是一种潜在的膳食降脂成分。

The monomer lansiumamide B(LB)was obtained from wampee seeds by extraction,separation,and structural identification It was demonstrated through cell and mouse experiments that LB has a strong hypolipidemic effect in vivo and in vitro.The results showed that:LB could effectively inhibit the activities ofα-amylase,α-glucosidase,and pancreatic lipase,with IC 50 values of 18.43,15.27,and 0.16 mg/mL,respectively.Acting on oleic acid(OA)-induced high-fat HepG2 cells,when the LB dose was 5μg/mL,triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),alanine aminotransferase(ALT),aspartate aminotransferase(AST),and malondialdehyde(MDA)contents decreased by 30.77%,30.00%,58.33%,39.97%,38.84%,and 43.90%,respectively.However,high-density lipoprotein cholesterol(HDL-C),catalase(CAT),and low-density lipoprotein receptor(LDLR)contents increased by 83.33%,253.37%,and 60.97%,respectively.After 6 weeks of feeding high-fat mice with LB,their body weight significantly decreased compared with that of the high-fat control group(P<0.05).When a 60 mg/kg LB dose was provided,TC,TG,and LDL-C contents decreased by 29.18%,23.69%,and 70.27%,respectively,and HDL-C content increased by 50.51%.The results showed that the four indices of body weight and blood lipids of high-fat mice were all well-controlled.These results suggest that LB is effective at reducing blood lipids in cells and in vivo,and is a potential dietary hypolipidemic compound.