3D打印技术构建不同比例PLA/TiO_(2)复合支架及其性能评估

Construction and performance evaluation of PLA/TiO_(2) nanocomposite scaffolds with different proportions by 3D printing technology

目的利用3D打印技术构建不同比例的聚乳酸/二氧化钛(PLA/TiO_(2))纳米复合支架,探究其相关性能.方法在PLA中分别添加1%、1.5%、2.5%、5%和10%的TiO_(2)纳米颗粒.通过3D打印技术构建PLA/TiO2纳米复合支架,对其形貌、力学性能、孔隙率、热性能和生物性能进行表征.结果与纯PLA支架相比,PLA/TiO_(2)-1.5%、PLA/TiO_(2)-2.5%支架具有更高的孔隙率和更好的力学性能.所有支架均为层层交互叠加的网状结构,孔径约200μm,孔隙均匀规则.体外细胞增殖实验发现,所有支架均可促进小鼠胚胎成骨细胞增殖,PLA/TiO_(2)-1.5%组支架的细胞增殖效果优于其他组支架(P<0.05).结论相比于其他支架,PLA/TiO_(2)-1.5%支架具有适宜的力学性能,高孔隙率,更好地促进细胞增殖能力,可为骨缺损修复材料研究奠定基础.

Objective PLA/TiO_(2) nanocomposite scaffolds with different ratios were constructed by 3D printing technolo-gy,and their related properties were explored.Methods 1%,1.5%,2.5%,5% and 10% of TiO_(2) nanoparticles were added to polylactic acid(PLA),respectively.The PLA/TiO_(2) nanocomposite scaffolds were constructed by 3D printing technology,and their morphology,mechanical properties,porosity,thermal properties and biological properties were characterized.Results Compared with pure PLA scaffolds,PLA/TiO_(2)-1.5% and PLA/TiO_(2)-2.5% scaffolds had higher porosity and better mechanical properties.All scaffolds were layer by-layer reticular structures with a pore size of about 200 pm,and the pores were uniform and regular.In vitro cell proliferation experiments showed that all scaffolds could promote the proliferation of mouse embryonic osteoblasts,and the cell proliferation effect of scaffolds in PLA/TiO_(2)-1.5% group was better than that in scaffolds in other groups(P<0.05)。Conclusion Compared with other scaffolds,PLA/TiO_(2)-1.5% scaffolds have suitable mechanical properties,high porosity,and better ability to promote cell proliferation,which can lay a foundation for the research on bone de-fect repair materials.