三相分离法提取裂殖壶藻中油脂、蛋白质和多糖的工艺优化

Process optimization of three phase partitioning to extract the lipids,protein and polysaccharide from Schizochytrium sp.

为了高值化综合开发裂殖壶藻,以未破壁的裂殖壶藻粉为原料,优化三相分离(TPP)法联产提取裂殖壶藻中油脂、蛋白质和多糖的工艺。结果表明,采用TPP法联产提取裂殖壶藻中油脂、蛋白质和多糖的最佳条件为悬浮液中硫酸铵质量浓度5 g/15 mL,叔丁醇与悬浮液体积比3∶1,液料比120∶1。在上述优化的条件下,TPP法提取裂殖壶藻油脂的最佳提取温度和提取时间分别为90℃和3 h,提取蛋白质和多糖的最佳提取温度和提取时间分别为60℃和0.5 h。此外,评估不同策略下TPP法联产提取裂殖壶藻油脂、蛋白质和多糖的性能发现,先在60℃下提取0.5 h再在90℃下提取3 h,总油脂、蛋白质和多糖提取率最高,分别达97.86%、57.11%和28.15%,油脂中DHA含量可达35.30%,DPA含量可达5.53%。综上,TPP法是一种高效联产提取裂殖壶藻中油脂、蛋白质和多糖的方法。

In order to the comprehensive development of Schizochytrium sp.with high value,the process of the simultaneous extraction of oil,protein and polysaccharide from the unbroken Schizochytrium sp.by three phase partitioning(TPP)method were optimized.The results showed that the optimal conditions for the simultaneous extraction of oil,protein and polysaccharide from Schizochytrium sp.by TPP method were as follows:mass concentration of ammonium sulfate in suspension 5 g/15 mL,volume ratio of t-butanol to suspension 3:1,ratio of total volume to algal powder mass 120:1.Under the optimized conditions,the optimal extraction temperature and time of lipids from Schizochytrium sp.by TPP method were 90℃and 3 h,respectively,and the optimal extraction temperature and time of protein and polysaccharide were 60℃and 0.5 h,respectively.Moreover,the extraction performance of TPP method to simultaneously extract the lipids,protein and polysaccharide from Schizochytrium sp.was further evaluated by different strategies.It was found that the extraction at 60℃for 0.5 h followed by 90℃for 3 h resulted in the highest extraction rates of 97.86%,57.11%and 28.15%for total lipids,protein and polysaccharide,respectively,with DHA content up to 35.30%and DPA content up to 5.53%in the lipids.In conclusion,the TPP method is a highly efficient methodfor simultaneously extracting lipids,protein and polysaccharideefrom Schizochytrium sp.