胃腺癌组织RBMS1、AKAP12表达变化及其临床意义

Expression changes of RBMS1 and AKAP12 in gastric adenocarcinoma and their clinical significance

目的探讨胃腺癌组织RNA结合基序单链相互作用蛋白1(RBMS1)、A激酶锚定蛋白12(AKAP12)表达变化及其临床意义。方法选择胃腺癌患者102例,取手术切除的胃癌组织及其癌旁组织(距肿瘤组织边缘5 cm以上,经病理检查明确为正常胃组织),采用免疫组化法检测RBMS1、AKAP12表达。比较胃癌组织与癌旁正常组织RBMS1、AKAP12阳性表达率,采用Pearson线性相关分析法分析胃癌组织RBMS1阳性表达与AKAP12阳性表达的关系。分析胃癌组织RBMS1、AKAP12阳性表达与临床病理特征的关系以及二者表达与预后的关系。结果胃癌组织RBMS1阳性表达率显著高于癌旁正常组织,AKAP12阳性表达率显著低于癌旁正常组织(χ^(2)分别为75.583、53.204,P均<0.05)。胃癌组织RBMS1阳性表达与AKAP12阳性表达呈负相关关系(r=-0.625,P<0.05)。胃癌组织RBMS1、AKAP12阳性表达与TNM分期、淋巴结转移有关(P均<0.05),与性别、年龄、肿瘤最大径、肿瘤部位、组织分化程度无关(P均>0.05)。所有患者术后随访2~36个月,中位随访时间25.30个月。随访期间失访1例,死亡30例,3年总体生存率为70.30%(71/101)。胃癌组织RBMS1阳性表达者与阴性表达者3年总体生存率分别为66.20%(47/71)、80.00%(24/30),胃癌组织AKAP12阳性表达者与阴性表达者3年总体生存率分别为82.14%(23/28)、65.75%(48/73)。胃癌组织RBMS1阳性表达者3年总体生存率显著低于其阴性表达者(χ^(2)=4.310,P<0.05),胃癌组织AKAP12阳性表达者3年总体生存率显著高于其阴性表达者(χ^(2)=4.569,P<0.05)。结论胃腺癌组织RBMS1高表达、AKAP12低表达,二者表达变化与肿瘤TNM分期、淋巴结转移和患者预后密切相关。

Objective To investigate the expression changes of RNA-binding motif single-stranded interacting pro⁃tein 1(RBMS1)and A kinase anchor protein 12(AKAP12)in gastric adenocarcinoma and their clinical significance.Methods Totally 102 patients with gastric adenocarcinoma were selected.Gastric cancer tissues and adjacent tissues(more than 5 cm away from the tumor tissue margin,which were confirmed as normal gastric tissues by pathological exami⁃nation)were removed,and the expression levels of RBMS1 and AKAP12 were detected by immunohistochemical method.The positive expression rates of RBMS1 and AKAP12 in the gastric cancer tissues and adjacent normal tissues were com⁃pared.Pearson linear correlation analysis was used to analyze the relationship between the positive expression of RBMS1 and AKAP12 in gastric cancer tissues.We analyzed the relationships between positive expression of RBMS1 and AKAP12 and clinicopathological features and prognosis of gastric cancer.Results The positive expression rate of RBMS1 in the gastric cancer tissues was significantly higher than that in paracancer normal tissues,and the positive expression rate of AKAP12 was significantly lower than that in the paracancer normal tissues(χ^(2)=75.583 and 53.204,both P<0.05).The positive expression of RBMS1 was negatively correlated with the positive expression of AKAP12 in gastric cancer(r=-0.625,P<0.05).The positive expression of RBMS1 and AKAP12 in gastric cancer tissues was related to TNM stage and lymph node metastasis(all P<0.05),but not to gender,age,maximum tumor diameter,tumor site or degree of tissue dif⁃ferentiation(all P>0.05).All patients were followed up for 2 to 36 months,with a median follow-up time of 25.30 months.During follow-up,one case was lost to follow-up and 30 died.The 3-year overall survival rate was 70.30%(71/101).The 3-year overall survival rates of RBMS1 positive and negative patients were 66.20%(47/71)and 80.00%(24/30),respectively.The 3-year overall survival rates of AKAP12 positive and negative patients were 82.14%(23/28)and 65.75%(48/73),respectively.The 3-year overall survival rate in patients with positive expression of RBMS1 was signifi⁃cantly lower than that in patients with negative expression of RBMS1(χ^(2)=4.310,P<0.05),and the 3-year overall survival rate in patients with positive expression of AKAP12 was significantly higher than that in patients with negative expression of AKAP12(χ^(2)=4.569,P<0.05).Conclusion RBMS1 is highly expressed and AKAP12 is low expressed in the gastric adenocarcinoma tissues,and their expression changes are closely related to TNM staging,lymph node metastasis,and prognosis of patients.