新疆葡萄酒对大鼠肝脏功能及肝细胞自噬的影响

Effects of Xinjiang wine on liver function and hepatocyte autophagy in rats

该实验为了探讨新疆葡萄酒对肝脏及肝细胞自噬的影响,将雄性SD大鼠随机分为6组:空白组(1)、酒精组(2)、吐哈盆地干白葡萄酒组(3)、天山北麓干红葡萄酒组(4)、伊犁河谷干红葡萄酒组(5)、焉耆盆地干红葡萄酒组(6),用新疆不同产区葡萄酒灌胃雄性SD大鼠8周后,检测6组大鼠体重变化和肝脏系数、肝脏组织病理形态学变化、血清中谷丙转氨酶(alanine transaminase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)含量,并通过Western blotting检测肝脏组织中自噬相关蛋白LC3、p62表达水平。结果表明,与空白组相比,3、4、5、6葡萄酒组大鼠体重增长趋势较缓,酒精组大鼠体重增长趋势最缓,与酒精组相比,3、4、5、6葡萄酒组肝脏系数显著降低(P<0.05),肝脏未见明显病变,大鼠血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、AST、ALT、血尿素氮(blood urea nitrogen,BUN)、血肌酐(serum creatinine,SCr)、总蛋白(total protein,TP)、白蛋白(albumin,ALB)、血糖(blood glucose,GLU)呈降低趋势,高密度脂蛋白胆固醇(high density lipoproteincholesterol,HDL-C)有上升趋势(P>0.05),球蛋白(globulin,GLOB)含量无显著差异,LC3蛋白含量升高,p62蛋白含量降低,且都达到了显著水平(P<0.05)。机体适量摄入葡萄酒一定程度上能降低肝脏系数、调节大鼠体内血清理化指标及肝脏功能、促进肝细胞自噬水平,从而减少酒精对肝脏的损伤,这可能与葡萄酒中含有的酚类物质有关。

To investigate the effect of Xinjiang wine on autophagy of liver and hepatocytes,male SD rats were randomly divided into six groups,including blank group(1),alcohol group(2),Tuha Basin dry white wine group(3),the northern foothill of Tianshan Mountain dry red wine group(4),Yili River Valley dry red wine group(5),and Yanqi Basin dry red wine group(6).After 8 weeks of gavage with Xinjiang wine from different producing areas,the changes of body weight,liver coefficient,pathomorphological changes of liver tissue,the contents of alanine transaminase(ALT)and aspartate aminotransferase(AST)in serum,and the expression levels of autophagy related proteins LC3 and p62 in liver were detected by Western blotting.Results showed that compared with the blank group,the weight growth trend of rats in wine group 3,4,5,and 6 was slower,and the weight growth trend of rats in alcohol group was the slowest.Compared with alcohol group,the liver coefficient decreased significantly(P<0.05),and there was no obvious lesion in the liver.Total cholesterol,triglyceride,low density lipoprotein cholesterol,AST,ALT,blood urea nitrogen,serum creatinine,total protein,albumin,globulin,and blood glucose in serum decreased,high density lipoprotein cholesterol has an upward trend(P>0.05),and the content of globulin has no significant difference.LC3 protein content increased and p62 protein content decreased,and all reached a significant levels(P<0.05).Moderate intake of wine can reduce the liver coefficient,regulate the blood clearance index and liver function in rats,and promote the level of hepatocyte autophagy,so as to reduce the damage of alcohol to the liver,which may be related to the phenolic substances contained in wine.