生脉散对2型糖尿病模型大鼠糖脂代谢和肠道菌群的影响

Effect of Shengmai San on glucolipid metabolism and intestinal flora in type 2 diabetes model rats

目的探索生脉散对2型糖尿病(T2DM)模型大鼠糖脂代谢和肠道菌群的影响。方法采用腹腔注射低剂量链脲佐菌素(STZ)联合高糖高脂饲料喂养的方法,建立T2DM胰岛素抵抗大鼠模型。将大鼠分为正常组、模型组、二甲双胍组、辛伐他汀组、生脉散低、中、高剂量组,连续给药6周后,检测其空腹血糖(FBG)、空腹胰岛素(FINS)、糖化血红蛋白(HbAlc)水平,以及总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)的水平;并采用16srDNA高通量测序检测大鼠肠道菌群组成和结构,采用高效液相色谱法检测大鼠粪便中短链脂肪酸(SCFAs)含量变化情况。结果生脉散各剂量组均可降低2型糖尿病大鼠FBG、FINS、HbAlc等血糖指标(P<0.01或0.05),降低血脂指标中TC、TG、LDL-C的水平(P<0.01),升高HDL-C的水平(P<0.01)。此外,生脉散的干预,可以恢复模型组的OUTs数目,提高Chao1、Shannon与Simpson指数(P<0.01),升高厚壁菌门、拟杆菌门和疣微菌门的丰度(P<0.01),降低放线菌门和变形菌门的丰度(P<0.01或0.05),并促进SCFAs的分泌。结论生脉散可改善T2DM模型大鼠糖脂代谢,其机制可能与改善肠道菌群的组成和结构有关。

Objective To investigate the effect of Shengmai San on glucolipid metabolism and intestinal flora in type 2 diabetes model rats.Methods An insulin-resistant type 2 diabetic rat model was established with the method of intraperitoneal injection of low-dose streptozotocin(STZ)combined with high-sugar and high-fat feed.The rats were divided into the Control Group,the Model Group,the Metformin Group,the Simvastatin Group,and Shengmai San Low-Dose,Medium-Dose and High-Dose Groups.After 6 weeks of continuous administration,the fasting blood glucose(FBG),fasting insulin(FINS),and glycosylated hemoglobin(HbAlc)levels as well as total cholesterol(TC),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(LDL-C)levels were detected.The composition and structure of intestinal flora of rats were detected with 16s rDNA high-throughput sequencing,and the content of short-chain fatty acids(SCFAs)in feces of rats was detected by high performance liquid chromatography.Results In all Shengmai San groups,the FBG,FINS,HbAlc and other blood glucose indexes of type 2 diabetic rats were all decreased(P<0.01 or 0.05),the TC,TG and LDL-C levels among blood lipid indexes were all reduced(P<0.01),and the HDL-C level was increased(P<0.01).In addition,the intervention of Shengmai San could restore the number of OUTs in the Model group,increase the Chao1,Shannon and Simpson indexes(P<0.01),increase the abundance of firmicutes,bacteroidetes and warthomicrophyla(P<0.01),reduce the abundance of actinobacteria and proteobacteria(P<0.01 or 0.05),and promote the secretion of SCFAs.Conclusion Shengmai San can improve glucolipid metabolism in type 2 diabetes rats,and the mechanism may be related to improvement of composition and structure of intestinal flora.