单一内生真菌与混合内生真菌对杉木幼苗生长及光合作用的影响

Effects of Single Endophytic Fungi and Mixed Endophytic Fungi on Growth and Photosynthesis of Cunninghamia lanceolata Seedlings

【目的】探究单一内生真菌和混合内生真菌对杉木幼苗生长及光合作用的影响。【方法】将从生长健壮、无病虫害的杉木根、茎和叶片样品中分离出的内生真菌,以杉木020号无性系幼苗作为接种苗木,接种促生菌以及混合菌,测定杉木幼苗生长特性和光合作用生理指标。【结果】4株内生真菌螺旋木霉菌(S10)、炭团菌(S22)、胶孢炭疽菌(S24)和二型伞霉菌(S38)在杉木幼苗根部定殖成功。通过分析苗高地径、地上地下部分生物量和根冠比等生长特性指标可知:单株菌S24和混合菌S10×S22、S22×S38在促进杉木幼苗苗高和地径生长作用显著,相对于CK组,单株菌S24对杉木幼苗苗高和地径的增长率分别为2.99%和15.66%,混合菌对杉木幼苗苗高和地径的增长率分别为:S10×S22(13.81%和3.04%)和S22×S38(18.99%和11.01%)。单株菌S22、S24和S38的地上和地下部分生物量相对于CK组增长率分别为:S22(22.86%和58.57%)、S24(18.94%和53.74%)和S38(15.87%和57.40%)。分析叶绿素含量、叶绿素荧光参数(F_(0)、F_(v)/F_(m))等光合特性指标可知:单株菌S38和混合菌S10×S38、S22×S24在45~60 d阶段显著提高杉木幼苗叶绿素含量,相对于CK组,S38增长率为11.15%和13.70%,混合菌增长率分别为:S10×S38(2.25%和17.53%)和S22×S24(10.72%和13.51%);接种单株菌S24和混合菌S22×S38的杉木幼苗相比较于其他菌株具有更低的F_(0)值;F_(v)/F_(m)值始终处于0.80~0.85的范围中。【结论】单一内生真菌和混合内生真菌都对杉木幼苗生物量、光合作用有显著的提升,可以有效促进杉木幼苗生长,混合内生真菌的促生效果要高于单一内生真菌。

【Objective】 This study purposes to explore the effects of single endophytic fungus and mixed endophytic fungi on the growth and photosynthesis of Cunninghamia lanceolata seedlings.【Method】 The endophytic fungi isolated from the root,stem and leaf samples of Chinese fir with robust growth and no diseases and insect pests were used as inoculated seedlings.The growth characteristics and photosynthetic physiological indexes of C.lanceolata seedlings were determined by inoculating the single or mixed growth promoting fungi.【Result】 Four endophytic fungi Trichoderma spirale(S10),Hypoxylon(S22),Colletotrichum gloeosporioides(S24) and Umbelopsis dimorpha(S38) were successfully colonized in the root of C.lanceolata seedlings.Through the analysis of seedling height diameter,aboveground and underground biomass,root to shoot ratio and other growth characteristics,it can be seen that:single strain S24and mixed strains S10×S22 and S22×S38 significantly promoted the growth of seedling height and ground diameter of C.lanceolata seedlings.Compared with CK group,the growth rates of single strain S24 on seedling height and ground diameter of C.lanceolata seedlings were 2.99% and 15.66%,respectively.The growth rates of seedling height and ground diameter for mixed strains were 13.81% and 3.04%,respectively,for S10×S22,and 18.99% and 11.01%,respectively,for S22×S38.The aboveground and underground biomass growth rates of individual strains S22,S24 and S38 were S22(22.86% and 58.57%)、S24(18.94% and 53.74%)and S38(15.87% and 57.40%),respectively,compared with CK group.By analyzing chlorophyll content,chlorophyll fluorescence parameters(F_(0)、F_(v)/F_(m)) and other photosynthetic characteristics,it can be seen that:single strain S38 and mixed strains S10×S38 and S22×S24 significantly increased the chlorophyll content of seedlings at 45-60 d stage.Compared with CK group,chlorophyll content of C.lanceolata in S38 and CK group were 11.15%higher.The growth rates of mixed strains were as follows:S10×S38(2.25% and 17.53%)and S22×S24(10.72% and 13.51%).C.lanceolata seedlings inoculated with single strain S24 and mixed strain S22×S38 had a lower F0 value than that inoculated with other strains.The F_(v)/F_(m) value ranged from 0.80 to 0.85 across all treatments.【Conclusion】 These results indicated that both single endophytic fungus and mixed endophytic fungus inoculations significantly improved the biomass and photosynthesis of C.lanceolata seedlings,and could effectively promote the growth of C.lanceolata seedlings.The growth promotion effect of mixed endophytic fungi was stronger than that of single endophytic fungus.