褪黑素浸种对滇油杉种子发芽及苗木生长和生理特性的影响

Effect of Melatonin on Seed Germination and Seedling Growth and Physiological Properties of Keteleeria evelyniana

【目的】了解褪黑素浸种对滇油杉(Keteleeria evelyniana)种子发芽和苗木生理的影响,开展不同浓度褪黑素溶液浸种试验,为滇油杉种子发芽及壮苗培育提供科学理论依据。【方法】采用单因素完全随机试验设计,开展不同浓度(0、0.3、0.6、0.9和1.2 mmol/L)褪黑素溶液浸种研究。计算处理的种子发芽性状,以及测定苗木生长、光合色素及酶活性的变化。【结果】处理的发芽率、发芽势、平均发芽时间、发芽指数、叶绿素、类胡萝卜素含量、SOD、POD、CAT活性和MDA含量分别为40.4%~60.0%、14.5%~32.1%、12.6~14.6 d、4.2~7.2粒/d、0.96~1.43 mg/g、0.16~0.23 mg/g、185.4~360.7 U/(g·min)、24.4~64.9 U/(g·min)、1532.1~1916.3 U/(g·min)、9.5~25.6 nmol/g,褪黑素处理的所有指标均显著或极显著地优于对照的(P<0.05或P<0.01)。【结论】0.3 mmol/L褪黑素溶液浸种有益于种子发芽、光合色素形成和提高保护酶的活性,促进苗木生长,可应用于滇油杉的种子处理。

【:Objective】In order to understand the effects of seed soaking with the melatonin on seed germination and seedling physiology of Keteleeria everyniana,this study implemented seed soaking trial with different concentrations of melatonin solution to provide scientific and theoretical basis for seed germination and strong seedling cultivation of K.everyniana【.Method】A single factor complete randomized experiment design was employed to carry out the trial of seed soaking with different concentrations of melatonin solutions.Seed germination properties were recorded among treatments,and the changes of seedling growth,photosynthetic pigments and enzyme activities were measured.【Result】The germination rates,germination potentials,mean germination time,germination index,chlorophyll content,carotenoid content,activities of SOD,POD,CAT and MDA content were 40.4%−60.0%,14.5%-32.1%,12.6−14.6 day,4.2−7.2 seed/day,0.96−1.43 mg/g,0.16−0.23 mg/g,185.4−360.7 U(/g·min),24.4−64.9 U(/g·min),1532.1−1916.3 U(/g·min)and 9.5−25.6 nmol/g,respectively,with all parameters of the melatonin-treated seeds or seedlings significantly higher than those of control(P<0.05 or P<0.01).【Conclusion】Soaking seeds with 0.3 mmol/L melatonin solution was found to be beneficial for seed germination,photosynthetic pigment formation,improving the activities of protective enzymes,and promoting the growth of seedlings,which could be applied to seed treatment of K.everyniana.