玉米bZIP基因应答逆境胁迫的表达模式分析

The Expression Profiling of bZIP Genes under Adverse Stress in Maize

【目的】碱性亮氨酸拉链蛋白(b ZIP)是植物中普遍存在而又关键的一类转录因子,在植物生长进程中发挥重要的作用。探测玉米核心种质自交系郑58不同组织以及在不同胁迫条件下bZIP基因的表达模式,对解析ZmbZIP基因的功能及抗性育种具有重要意义。【方法】通过设置200 mmol/L的NaCl溶液、20%PEG6000、4℃低温和不同形态氮缺乏胁迫试验,试验材料为玉米骨干自交系郑58,采用q RT-PCR技术,共检测了11个ZmbZIP基因的表达模式。【结果】进化树结果表明11个ZmbZIP可以进一步划分3个亚家族,显示这11个基因的进化亲缘关系。qPCR分析数据表明,11个ZmbZIP基因在不同组织器官中表达模式各异,表明其在玉米生长进程中的不同作用。利用200 mmol/L NaCl、20%PEG6000、4℃低温和氮缺乏等模拟试验,研究了11个ZmbZIP基因的表达谱,结果表明4种非生物胁迫都显著的影响11个bZIP基因表达,推测这些基因参与维持玉米正常生长发育及逆境胁迫应答信号途径。【结论】在玉米不同组织器官和响应非生物胁迫时,11个ZmbZIP基因表达谱差异比较大,预示着生物学功能的差异化,为将来探究这些基因的生理生化作用提供科学依据。

【Objective】 Basic leucine zipper(bZIP) proteins constitute a large family of transcription factors among plants,which play vital roles during plant development.the expression of 11 ZmbZIPs in different tissues or under various stresses in elite maize inbred line Zheng58 could provide reference for the functional study of ZmbZIP or for the resistance breeding.【Method】 Using qRT-PCR technique,this paper detected the expression profiling of 11 ZmbZIPs of maize inbred line Zheng58 was detected under 200 mmol/L NaCl,20% PEG6000,4 ℃ low temperature and nitrogen dificiency stresses.【Result】 Plylogenetic tree revealed that 11 ZmbZIPs can be subdivided into 3 subgroups,indicating their genetic relationships.RT-qPCR analysis showed that 11 ZmbZIPs exhibited different expression patterns in different tissues,indicating diverse roles during maize growth and development.Under simulated stress treatment,200 mmol/L NaCl,20% PEG6000,4 ℃ and nitrogen deficiency,the expression patterns of 11 ZmbZIPs were detected,and the results showed that 11 ZmbZIPs were regulated by 4 stress treatments,indicating it's widely involved in stress signaling pathways.【Conclusion】 The expression patterns of 11 ZmbZIPs differed in different tissues or in responsive to various stresses.The study can offer valuable information for further revealing its biological functions.