芥菜全基因组中Hsf基因家族鉴定与分析

Genome-Wide Identification and Analysis of the Heat Shock Transcription Factor Family in Brassica juncea L.

【目的】通过鉴定与分析芥菜基因组中Hsf转录因子家族成员,为芥菜Hsf基因功能研究与遗传改良提高抗逆性提供理论依据。【方法】利用生物信息学方法分析芥菜Hsf热激转录因子家族成员的功能结构、保守基序、启动子顺式作用元件、系统进化、共线性及采用RNA-seq验证Hsf低温胁迫的基因表达。【结果】芥菜基因组中鉴定出71个Hsf基因家族成员,分布于18条染色体上,归类为3个亚家族,蛋白均含有DBD和HR-A/B结构域。BjuHsf启动子区域包含与逆境胁迫、激素、生长发育等相关顺式作用元件。系统进化与共线性分析表明,芥菜Hsf家族成员与大白菜Hsf家族成员具有更近的亲缘关系。在低温胁迫下,BjuHsf基因表达分析表明8个BjuHsf基因显著上调表达。【结论】这些显著差异表达BjuHsf基因与芥菜抗寒性极其相关,可作为芥菜耐寒性遗传改良的候选基因。

【Objective】 This paper aims to identify and analyze the members of Hsf transcription factor family in Brassica juncea L.genome,hoping to provide a theoretical basis for the functional research of Brassica juncea L.Hsf gene and genetic improvement to improve stress resistance.【Method】 Bioinformatics methods were used to analyze the functional structure,conserved motifs,promoter cis acting elements,phylogeny,collinearity of BjuHsf family members,and RNA-seq was also used to verify the gene expression of BjuHsf under low temperature stress.【Result】 Seventy-one members of the Hsf gene family were identified in the Brassica juncea L.genome,distributed on eighteen chromosomes and grouped into three subfamilies.all BjuHsf proteins contain DBD and HR-A/B domains.The BjuHsf promoter region contains cis-acting elements related to stress,hormones,and growth and development.The phylogenetic and collinearity analysis indicated that the Hsf family members of Brassica juncea L.were more closely related to those of chinese cabbage.Under low temperature stress,BjuHsf gene expression analysis showed that eight BjuHsf genes were significantly up-regulated.【Conclusion】 These differentially expressed BjuHsf genes are related to cold tolerance of Brassica juncea L.and can be used as candidate genes for genetic improvement of cold tolerance of Brassica juncea L.