糖基化β-伴大豆球蛋白负载提高姜黄素抗氧化及缓释特性

Enhanced Antioxidant Activity and Sustained-Release Property of Curcumin Encapsulated in Glycated Soyβ-Conglycinin Nanoparticles

利用干法糖基化制备β-伴大豆球蛋白(soyβ-conglycinin,7S)-葡聚糖共价接枝物包埋姜黄素,并研究姜黄素的抗氧化及缓释效果。结果表明,随着反应时间(0、24、48、72 h)延长,7S-葡聚糖共价复合物接枝度和褐变度逐渐增加,至72 h达到最高,分别为15.02%和0.24。通过pH值循环法诱导7S、7S-葡聚糖封装姜黄素,形成7S-姜黄素、7S-葡聚糖-姜黄素纳米复合物,发现反应时间为72 h时,7S-葡聚糖表现出最高的姜黄素负载量(129.61μg/mg),显著高于7S(69.06μg/mg)。荧光光谱分析表明姜黄素主要通过疏水相互作用与7S、7S-葡聚糖-72 h结合。透射电子显微镜观察到7S-姜黄素和7S-葡聚糖-72 h-姜黄素纳米复合物为球形。姜黄素质量浓度为100μg/mL时,与游离的姜黄素相比,封装在7S-葡聚糖-72 h内的姜黄素抗氧化能力提高1.36倍,且表现出稳定的缓释性能。7S-葡聚糖-72 h负载的姜黄素生物可利用率为66.83%,而7S负载的姜黄素和游离姜黄素生物可利用率分别为48.93%和33.06%。研究结果表明7S-葡聚糖-72 h可作为良好的姜黄素纳米载体,改善其抗氧化及缓释能力。

Soyβ-conglycinin(7S)-dextran conjugates prepared under dry-heating conditions were used to encapsulate curcumin,and the antioxidant activity and sustained-release property of encapsulated curcumin were investigated.Results showed that the grafting degree and browning index of 7S-dextran conjugates increased with increasing reaction time(0,24,48 and 72 h),reaching the highest values of 15.02%and 0.24 at 72 h,respectively.7S-curcumin and 7S-dextran-curcumin nanocomplexes were fabricated by pH-shifting method.The highest curcumin loading of 129.61μg/mg was observed in 7S-dextran conjugate obtained at 72 h of reaction time(7S-dextran-72 h),which was significantly higher than that in 7S(69.06μg/mg).Fluorescence spectroscopy indicated that curcumin combined with 7S or 7S-dextran-72 h mainly through hydrophobic interactions.It was observed by transmission electron microscopy(TEM)that 7S-curcumin and 7S-dextran-72 h-curcumin nanocomplexes were spherical.The antioxidant activity of 7S-dextran-72 h-curcumin,which had stable sustained-release property,was approximately 2.36 times higher than that of free curcumin at 100μg/mL.The bioavailability of curcumin encapsulated in 7S-dextran-72 h was 66.83%,while the bioavailability of curcumin encapsulated in 7S and free curcumin were 48.93%and 33.06%,respectively.7S-dextran-72 h could be used as an outstanding nanocarrier for curcumin to enhance its antioxidant activity and sustained-release property.