基于UPLC-MS/MS和GC-IMS技术分析采前套袋与未套袋油㮈果实品质差异

Comparative Analysis of the Effect of Pre-Harvest Bagging and Non-Bagging Treatment on Fruit Quality of‘Younai’Plum by UPLC-MS/MS and GC-IMS

采用超高效液相色谱-串联质谱(ultra-high performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)和气相-离子迁移谱(gas chromatography-ion mobility spectrometry,GC-IMS)技术结合多元统计分析方法,分析采前套袋对油㮈果实代谢产物、代谢途径以及挥发性成分的影响。结果表明,采前套袋后油㮈果皮和果肉着黄色。基于UPLC-MS/MS技术鉴定到604个油㮈果实代谢物,采前套袋改变果实代谢特征和代谢途径,筛选出显著差异代谢物58个,上调物质22个,下调物质36个;其中,与滋味相关的糖类和有机酸等物质含量差异不显著,而与滋味相关的氨基酸部分差异显著。基于GC-IMS技术鉴定到油㮈果实含有醛类14种、酯类5种、醇类4种、酮类3种和其他1种共27个挥发性物质,多数酯类物质在未套袋果实中含量较高,多数醇类物质在套袋果实中含量较高。采前套袋与未套袋油㮈果实挥发性成分具有不同的指纹图谱。基于中药数据库和已鉴定代谢物查询到199个活性成分,根据筛选标准得到37个关键活性成分,其中,采前套袋果实的槲皮素含量下调。由此说明,采前套袋可提高油㮈果实外观品质,对果实滋味、香气和功效成分等部分有影响。

In this study,ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)and gas chromatography-ion mobility spectrometry(GC-IMS)combined with multivariate statistical analysis were used to analyze the effect of pre-harvest bagging on metabolites,metabolic pathways and volatile composition in‘Younai’plum fruit.The results showed that the peel and pulp of‘Younai’plum subjected to pre-harvest bagging treatment were yellow.By UPLC-MS/MS,604 metabolites were identified,pre-harvest bagging changed the metabolic characteristics and pathways,and 58 significantly differential metabolites were selected,22 and 36 of which were up-regulated and down-regulated,respectively.Among them,the contents of taste-related substances such as sugars and organic acids were not significantly different,and the contents of taste-active amino acids taste were significantly different between the bagging and non-bagging groups.By GC-IMS,27 volatile substances were identified,including 14 aldehydes,5 esters,4 alcohols,3 ketones and 1 other compounds.Most esters were more abundant in non-bagged fruits,while most alcohols were more abundant in bagged fruits.The fingerprints of volatile components in‘Younai’plum with pre-harvest bagging and non-bagging treatment were different.By searching against the Traditional Chinese Medicine System Pharmacology Database and comparing them with identified metabolites,199 active ingredients were identified,and 37 key active ingredients were obtained according to the screening criteria.Among them,only quercetin was down-regulated in‘Younai’plum after pre-harvest bagging treatment.In conclusion,pre-harvest bagging can improve the appearance quality,but it may also have an effect on the taste-active,aroma-active and functional components of‘Younai’plum.