UPLC法同时测定苦参功劳颗粒中苦参碱和盐酸小檗碱的含量

Determination of Matrine and Berberine Hydrochloride in Kushen Gonglao Granules by UPLC

建立超高效液相色谱法(UPLC)同时测定苦参功劳颗粒中苦参碱和盐酸小檗碱含量的方法。采用Acquity BEH C18(2.1 mm×100 mm, 1.7μm)色谱柱,以甲醇-0.02 mol/L磷酸二氢钾溶液(用磷酸调节pH值至3.0)为流动相,梯度洗脱,流速为0.2 mL/min,柱温为30℃,检测波长为214 nm。苦参碱和盐酸小檗碱分别在12.04~48.17μg/mL、13.18~52.71μg/mL浓度范围内与峰面积呈良好的线性关系,相关系数(r)分别为1.0000、0.9999;准确度考察中苦参碱和盐酸小檗碱平均加样回收率分别为99.6%(RSD=1.0%,n=6)、98.9%(RSD=1.0%,n=6)。该方法具有良好的专属性、精密度及准确度,且操作简便、环境友好,可用于苦参功劳颗粒的质量控制。

To establish a method for simultaneous determination of Matrine and Berberine Hydrochloride in Kushen Gonglao granules.Ultra Performance Liquid Chromatography(UPLC)method was adopted,The separation was performed on a Acquity BEH C18(2.1 mm×100 mm,1.7μm)with mobile phase consisted of methanol-0.02 mol/L Potassium dihydrogen phosphate solution(Adjust pH to 3.0 with phosphoric acid),gradient elutionat,the flow rate was 0.2 mL/min,the column temperature was 30℃,The detection wavelength was set at 214 nm.Matrine and Berberine Hydrochloride concentration showed a good relationship with the peak area in 12.04~48.17μg/mL(r=1.0000)、13.18~52.71μg/mL(r=0.9999);the average recoveries were 99.6%(RSD=1.0%,n=6)、98.9%(RSD=1.0%,n=6).The method has good specificity,precision and accuracy,and is easy to operate and friendly to the environment.It can be used for quality control of Kushen Gonglao granules.