硫酸新霉素可溶性粉中非法添加4种苯并咪唑类和3种大环内酯类抗寄生虫药物HPLC-PDA检测方法的建立

Determination of 4 Benzimidazoles and 3 Macrolides Antiparasitic Drugs Illegally Added in Neomycin Sulfate Soluble Powder by HPLC-PDA

建立了硫酸新霉素可溶性粉中非法添加7种抗寄生虫药物的HPLC-PDA检测方法。采用十八烷基键合硅胶为填充剂;以水-乙腈为流动相,梯度洗脱,流速1.0 mL/min;二极管阵列检测器,波长为245 nm。通过液相色谱保留时间、峰纯度检查和光谱相似度检查对非法添加物进行确证。结果表明,该色谱条件下,7种抗寄生虫药物间分离度良好,在3~80μg/mL浓度范围内线性关系良好(R2>0.9998),回收率在103.9%~105.8%之间,RSD≤2.5%,4种苯并咪唑类和3种大环内酯类的检测限分别为0.05 g/kg和0.3 g/kg。该方法操作简便、快速、灵敏度高,可用于硫酸新霉素可溶性粉中非法添加抗寄生虫药物的测定。

A method was established for the determination of seven antiparasitic drugs in neomycin soluble powder by the high-performance liquid chromatography with photo-diode detector(HPLC-PDA).C18 column was applied with mobile phase consisting of acetonitrile and water,gradient elution,at a flow rate of 1.0 mL/min.The detection wavelength was 245 nm.Retention time,peak purity test and spectrum similar test were utilized to identify the antiparasitic drugs.seven antiparasitic drugs were detected through the established method,which showed a good limits of detection(0.05 g/kg of benzimidazoles and 0.3 g/kg of macrolides)and linear range(3~80μg/mL)with a high correlation coefficients(r^(2)>0.9998).The recoveries ranged from 103.9%to 105.8%.The established method is simple,rapid and sensitive,and can be utilized for the determination of antiparasitic drugs illegally added to neomycin sulfate soluble powder.