南召辛夷SRAP遗传多样性分析及指纹图谱的构建

Genetic Diversity Analyses and Fingerprints Construction of Nanzhao Magnoliae Flos Using SRAP

利用毛细管电泳技术,鉴定SRAP(Sequence-related amplified polymorphisms)引物在34个南召辛夷种系中扩增条带的遗传多样性,聚类分析并构建DNA指纹图谱,为南召辛夷的种系鉴定和分子育种提供理论依据。结果表明:(1)5对SRAP引物共扩增出71条带,其中59条多态性的条带(83.1%),每对引物扩增出多态性位点为9~15条,平均为11.8条;(2)选用多态性高、鉴别能力强的2对引物构建了34份南召辛夷种系基于22个位点的DNA指纹图谱;(3)34份南召辛夷的遗传相似系数为0.58~0.97。UPGMA聚类结果显示,34个南召辛夷样品可分为6大类群,且与采摘地无直接关系。

The capillary electrophoresis is used for detecting the polymorphisms among 34 Nanzhao Magnoliae Flos(MF)lines amplified by SRAP primers.The genetic similarity coefficient and cluster analysis and DNA fingerprints are conducted,providing the theoretical basis for variety identification and molecular breeding.The results show that:(1)A total of 71 bands are amplified from 34 Nanzhao MF lines using five pairs of SRAP primers,among which 59 demonstrated polymorphisms(83.1%).Each pair of SRAP primer amplified 9~15 polymorphic bands,with an average of 11.8.(2)Based on the two pairs of primers with high polymorphism and strong discriminating ability,DNA fingerprints of 34 Nanzhao MF based on 22 loci are constructed.(3)The genetic similarity coefficient is between 0.58~0.97.UPGMA clustering results show that those lines can be classified into six groups,and there is no direct relationship between the groups and sample locations.