葛根素对实验性大鼠牙周炎炎症反应和骨吸收的影响

Effect of puerarin on inflammation and alveolar bone resorption in experimental rats with periodontitis

目的:研究葛根素对实验性牙周炎大鼠的炎症和牙槽骨吸收的影响。方法:选用7周龄Sprague Dawley大鼠36只,随机分为3组(n=12)。一组作为对照组,余下大鼠全麻下行左侧上颌第一磨牙牙颈部正畸结扎丝结扎,建立慢性牙周炎模型。3组大鼠采用灌胃给药14 d:对照组和牙周炎组给予等量生理盐水,葛根素组给予400 mg/kg浓度葛根素给药。最后一次给药24 h后麻醉大鼠,腹主动脉取血,离心取血清用于外周血中IL-4、IL-10、IL-6、IFN-γ含量的检测;再处死所有大鼠,部分大鼠分离左侧上颌骨,去除左上颌第一磨牙颊腭侧实验处新鲜牙龈组织,余下上颌骨组织用于Micro-CT的检测;部分大鼠左侧上颌骨标本取材后保留牙龈组织,制作切片用于HE染色检测。结果:HE染色显示,与牙周炎组相比,葛根素组炎性细胞浸润明显减少。ELISA分析显示,葛根素促进血清中IL-4和IL-10表达,降低IL-6和IFN-γ表达水平(P均<0.05)。Micro-CT显示与牙周炎组相比,葛根素显著减少牙槽骨吸收(P<0.05)。结论:葛根素给药可以抑制实验性大鼠牙周炎的炎症和牙槽骨吸收。

Objective:To investigate the effect of puerarin on inflammation and alveolar bone resorption in rats with experimental periodontitis.Methods:Thirty-six 7-week-old Sprague-Dawley rats were randomly divided into three groups(n=12).Twelve rats as the control group and the remaining rats were to establish a chronic periodontitis model ligated with orthodontic ligature wire on the cervical part of the left maxillary first molar under general anesthesia.All three groups were administered by gavage for 14 days:equal amounts of saline were given to the control and periodontitis groups,and 400 mg/kg concentration of puerarin was given to the puerarin group.All rats were anesthetized after 24 h of the last administration,blood was collected from the abdominal aorta,and the serum was centrifuged for the detection of IL-4,IL-10,IL-6,and IFN-γin peripheral blood,then all rats were executed,some rats separated from the left maxilla,fresh gingival tissue removed from the buccal-palatal side of the left maxillary first molar,and the remaining maxillary bone tissue used for the detection of Micro-CT;some rats subjected to the left maxillary bone specimens taken with the gingival tissues,used for HE staining detection.Results:HE staining showed that inflam-matory cell infiltration was significantly reduced in the puerarin group compared to the periodontitis group.ELISA analysis showed that puerarin promoted IL-4 and IL-10 expression and decreased IL-6 and IFN-γexpression levels in serum(P<0.05).Micro-CT showed that puerarin significantly reduced alveolar bone resorption compared to the periodontitis group(P<0.05).Conclusion:Puerarin may inhibit inflammation and alveolar bone resorption in experimental periodontitis rats.