NGAL基因对结直肠恶性肿瘤影响的Meta分析

Meta analysis of the effects of the NGAL gene on colorectal malignancies

目的NGAL(neutrophil gelatinase associated lipocalin)作为lipocalin家族成员中的一位,最开始是在人中性粒细胞中被研究发现的,与基质金属蛋白酶(Matrix Metalloproteinase,MMP)活性有紧密的相关性。在许多正常组织细胞中,均有NGAL的表达,但在很多恶性肿瘤的演变过程中出现异常表达。本研究应用Meta分析方法系统评价NGAL对结直肠癌的影响。方法检索PubMed、Embase、中国知网、万方、维普等数据库中采用免疫组化研究方法探究NGAL与结直肠癌表达关系的研究文献,95%置信区间(CI)和优势比(OR)作为效应指标,应用RevMan5.4软件对符合标准的纳入文献结果进行Meta分析。结果根据纳入及排除标准最终纳入5篇符合标准的文献,Meta分析显示:NGAL基因在结直肠中与对应的癌和癌旁组织中存在差异表达(OR=16.01,95%CI 9.85~26.02,P<0.01),有无淋巴结转移中NGAL基因差异表达(OR=2.75,95%CI 1.69~4.47,P<0.01),不同分化程度中NGAL差异表达(OR=2.81,95%CI 1.44~5.48,P<0.01),在肿瘤分期中差异表达(OR=4.05,95%CI 2.30~7.16,P<0.01)。结论NGAL与结直肠癌发生发展过程存在相关性,在结直肠癌的诊治、预防与评估中有一定价值。

Objective As a member of the lipocalin family,NGAL(neurophil gelatinase associated lipocalin)was first discovered in human neutrophils and has a close correlation with matrix metalloproteinase(MMP)activity.NGAL is expressed in many normal tissues and cells,but abnormal expression occurs in the evolution of many malignant tumors.In this study,Meta-analysis was used to systematically evaluate the effect of NGAL on colorectal cancer.Methods The research literature on the relationship between NGAL and colorectal cancer expression using immunohistochemical methods in PubMed,EMBASE,CNKI,Wanfang,VIP and other databases were retrieved.95%confidence interval(CI)and odds ratio(OR)were used as effect indicators,and the Revman 5.4 software conducts Meta-analysis on the results of the included literatures that meet the standards.Results According to the inclusion and excluly included,Meta analysis showed that there were differences in the expression of NGAL gene between colorectal tissues and corresponding adjacent tissues(OR=16.01,95%CI 9.85~26.02,P<0.01),there were differences in the expression of NGAL gene in lymph node metastasis(OR=2.75,95%CI 1.69~4.47,P<0.01),and there were differences in the expression of NGAL gene in different degrees of differentiation(OR=2.81,95%CI 1.44~5.48,P<0.01),it was differentially expressed in tumor stages(OR=4.05,95%CI 2.30~7.16,P<0.01).Conclusion NGAL is correlated with the occurrence and development of colorectal cancer,and has certain value in the diagnosis,treatment,prevention and evaluation of colorectal cancer.