藁本中黄酮类化合物的提取及体外抗氧化活性研究

Extraction of Flavonoids from Ligusticum and Its Antioxidant Activity in Vitro

采用单因素实验与响应面实验结合的方法,优化了藁本中黄酮类化合物的提取工艺,并进行了体外抗氧化实验。结果表明,最佳提取工艺条件为:提取时间为2.5 h,提取温度为83℃,乙醇体积分数为84%,液料比为35 L/g(乙醇溶液体积与藁本粉末样品质量之比);在最佳提取条件下,藁本黄酮得率达到(22.64±0.18)mg/g;藁本黄酮具有一定的抗氧化能力,对·DPPH的清除效果最好,对·OH的清除效果次之,对·O_(2)^(-)的清除效果最差;对Fe^(3+)具有一定的还原能力。

In this paper,the extraction process of flavonoids from Ligusticum was optimized by single factor test and response surface methodology,and the antioxidant experiment in vitro was carried out.The optimum extraction conditions were as follows:extraction time 2.5 h,extraction temperature 83℃,ethanol concentration 84%,liquid⁃solid ratio 35 L/g(the ratio of ethanol volume to Ligusticum jeholense powder sample mass).Under these conditions,the yield of flavonoids from Ligusticum was(22.64±0.18)mg/g.The results of antioxidant experiment in vitro showed that flavonoids from Ligusticum had certain antioxidant capacity.The clearing effect of·DPPH is the best,the clearing effect of·OH is the second,and the clearing effect of·O_(2)^(-)is the worst.It also has the ability to restore Fe^(3+).