睡莲花总黄酮对小鼠酒精肝损伤的防治作用研究

Preventive effects of total flavonoids from Nymphaea candida on alcoholic liver injury in mice

研究睡莲花总黄酮(NCTF)对急性酒精肝损伤(AALI)的保护作用及其机制。将小鼠按体重随机分为6组,即空白对照组、模型组、联苯双酯组(DDB,63 mg/kg)及NCTF组(50、100、200 mg/kg)。每日按设定剂量灌胃给药,正常组和模型组灌胃给与等量蒸馏水。每次给药0.5 h后,除正常组外,其余各组小鼠灌胃给予52°白酒(13 mL/kg),连续7 d。末次给药并禁食16 h后,取血清及肝组织,检测血清及肝组织相关指标,并进行病理组织学分析。与模型组比较,NCTF(50、100、200 mg/kg)能明显改善肝脏组织病理损伤;NCTF(200 mg/kg)能显著降低小鼠血清ALT、TG、TC含量以及肝组织MDA、TNF-α、IL-1β、IL-6水平(P<0.05),同时能明显升高肝组织GSH和SOD活性(P<0.05)。与模型组比较,NCTF(50、100、200 mg/kg)和DDB组(63 mg/kg)小鼠肝组织Nrf2、HO-1、NQO1 mRNA的表达水平显著增强(P<0.01),Keap1水平显著降低(P<0.05)。以上结果表明NCTF对小鼠AALI具有较好的防治作用,其机制可能与其抗氧化、抑制促炎因子表达控及Nrf2/HO-1信号的调控相关。

To investigate the protective effect and underlying of total flavonoids from Nymphaea candida(NCTF)against acute alcohol liver injury(AALI)and its mechanisms.Mice were randomly divided into 6 groups according to their body weights:normal group,model group,bifendate group(DDB,63 mg/kg)and NCTF groups(50,100,200 mg/kg).White wine(52°)was given by intragastric administration every day for 7 consecutive days.After the last administration and fasting for 16 h,the serum and liver tissues were collected for determination of indexes,and histopathological analysis was performed.Compared with the model group,NCTF(50,100,200 mg/kg)significantly improved the pathological damage of liver tissue;NCTF(200 mg/kg)significantly reduced the content of serum ALT,TG and TC in mice,and liver tissue MDA,TNF-α,IL-1β,L-6 levels(P<0.05),and at the same time,GSH and SOD activities in liver tissue were significantly increased(P<0.05).NCTF(50,100,200 mg/kg)markedly increased the relative mRNA expression level of Nrf2、HO-1、NQO1 compared with model group,whereas Keap1 decreased in the liver tissues(P<0.01).These results suggest that NCTF has a better preventive effect on AALI mice,and its mechanism may be related to its antioxidant,inhibition of proinflammatory factor expression,and regulation of Nrf2/HO-1 signal.