基于液相色谱质谱联用技术的儿童免疫性血小板减少症血浆代谢组学研究

Plasma Metabolomics of children immune thrombocytopenia based on Untargeted Liquid Chromatography–Mass Spectrometry

目的:基于LC-MS寻找儿童ITP潜在生物标记物。方法:采集患儿及健康儿童外周血进行LC-MS检测,通过统计学方法分析,经数据库鉴定代谢物结构。结果:LC-MS/MS检测获得原始数据,经主成分分析、层次聚类分析、秩和检验及HMDB数据库中匹配鉴定,再通过ROC曲线分析,最终筛选鉴定出5种潜在生物标志物:间甲酚、腐胺、5-羟色胺、2-氧代精氨酸、精氨基琥珀酸。结论:检测鉴定出的5种关键差异性代谢物可能可以作为鉴别ITP患儿和健康儿童的临床实验室生物标记物。

Objective:To find the potential biomarkers of ITP based on LC-MS.Methods:The peripheral blood of the patient and healthy children was collected for LC-MS detection,and the metabolite structure was identified by database.Results:The original data obtained by LC-MS/MS were identified by principal component analysis,hierarchical cluster analysis,rank sum test and HMDB database matching,and then by ROC curve analysis,5 potential biomarkers were finally screened and identified:m-cresol,putreamine,5-hydroxytryptamine,2-oxo-arginine,argininosuccinic acid.Conclusionl:The five key differential metabolites identified may be used as clinical laboratory biomarkers to distinguish ITP children from healthy children.