外周血IL-6基因多态性与慢性阻塞性肺疾病曲霉菌感染易感性的关联

Association of peripheral blood IL-6 gene polymorphisms with susceptibility to Aspergillus fungal infection in patients with chronic obstructive pulmonary disease

目的分析外周血白细胞介素-6(IL-6)基因多态性与慢性阻塞性肺疾病(COPD)曲霉菌感染易感性的关联。方法选择2018年1月-2021年11月河北省中医院收治的67例单纯COPD患者为对照组、33例COPD并发曲霉菌感染患者为研究组。采用SNaPshot技术检测两组患者外周血IL-6基因单核苷酸多态性(SNPs)位点rs1800795(-174G/C)、rs1800796(-572C/G)、rs1800797(-597G/A)多态性,分析外周血IL-6基因多态性与COPD并发曲霉菌感染易感性及肺功能的关系。结果研究组和对照组患者外周血IL-6基因-174G/C、-597G/A位点基因型、等位基因分布频率比较,无统计学差异,-572C/G位点基因型、等位基因分布频率比较,差异有统计学意义(P<0.05);Logistic回归分析结果显示,携带GG基因型和G等位基因是影响COPD并发曲霉菌感染的危险因素(P<0.05);不同肺功能COPD并发曲霉菌感染患者轻中度和重度患者外周血IL-6基因-572C/G位点基因型、等位基因分布比较,无统计学差异,重度患者外周血IL-6水平高于轻中度患者(P<0.05)。结论外周血IL-6基因-572C/G位点多态性与COPD患者曲霉菌感染易感性有关,G等位基因为易感基因。

OBJECTIVE To observe the association between the peripheral blood interleukin-6(IL-6)gene polymorphisms and the susceptibility to Aspergillus fungal infection in patients with chronic obstructive pulmonary disease(COPD).METHODS A total of 67 patients with simple COPD who were treated in Hebei Hospital of Traditional Chinese Medicine from Jan 2018 to Nov 2021 were assigned as the control group,and 33 COPD patients who were complicated with Aspergillus fungal infection were assigned as the study group.The polymorphisms at rs1800795(-174G/C),rs1800796(-572C/G)and rs1800797(-597G/A)loci of peripheral blood IL-6 gene single nucleotide polymorphisms(SNPs)were detected for the two groups of patients by using SNaPshot technology,and the relationship between the peripheral blood IL-6 gene polymorphisms and the susceptibility to Aspergillus fungal infection and lung function of the COPD patients was observed.RESULTS There were no significant differences in the frequencies of genotypes and alleles at peripheral blood IL-6 gene-174G/C locus and-597G/A locus between the study group and the control group;there were significant differences in the frequencies of genotypes and alleles at-572C/G locus between the two groups(P<0.05).The result of logistic regression analysis showed that the GG genotype and allele G were the risk factors for the Aspergillus fungal infection in the COPD patients(P<0.05).Among the COPD patients complicated with Aspergillus fungal infection who had different lung function,there were no significant differences in the genotypes and alleles at peripheral blood IL-6 gene-572C/G locus between the mild and moderate patients and the severe patients;the peripheral blood IL-6 level of the severe patients was higher than that of the mild and moderate patients(P<0.05).CONCLUSION The polymorphism at peripheral blood IL-6 gene-572C/G locus is associated with the susceptibility to Aspergillus fungal infection in the COPD patients,and the allele G is a susceptibility gene.