复合菌肥对二氯喹啉酸胁迫下烟株抗氧化酶活性及丙二醛含量的影响

Effect of Compound Microbial Fertilizer on Antioxidant Enzyme Activities and Malondialdehyde Content of Tobacco under Quinclorac Stress

以烤烟K326为材料,采用漂浮育苗,于8叶期灌施富含多种微量元素和活性物质的复合菌肥,研究其对二氯喹啉酸胁迫下烟株抗氧化酶活性及丙二醛(MDA)含量的影响。设置5个处理,即CK(正常土壤)、T0(受害土壤)、T1(受害土壤施加复合菌肥1,干土中施入0.7 mL/kg)、T2(受害土壤施加复合菌肥2,干土中施入0.7 mL/kg)、T3(受害土壤施加复合菌肥3,干土中施入1.1 g/kg)。结果表明,受二氯喹啉酸危害(T0)的烟叶中超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)及过氧化氢酶(CAT)活性分别比正常烟株(CK)低27.74%、30.43%和39.67%,膜脂过氧化产物MDA含量升高213.98%。T1、T2、T3处理20 d的烟叶SOD活性分别比T0增加16.60%、18.16%、30.61%,APX活性分别升高11.48%、16.40%、33.35%,CAT活性分别增加25.23%、27.92%、40.54%,MDA含量分别降低8.22%、30.82%、59.25%。根系3种酶活性变化与MDA含量变化效果一致。各处理效果依次为CK>T3>T2>T1>T0,因此通过添加适量的复合菌肥可缓解二氯喹啉酸对烟株的危害。

This paper studied the effect of compound microbial fertilizer on antioxidant enzyme activity and malondialdehyde(MDA)content of flue-cured tobacco leaves damaged by quinclorac stress.Flue-cured tobacco K326 seedlings were experimental material using floating seedling and compound microbial fertilizer which riched in various microelements and active substances was applied at 8-leaf stage.The experiment set five treatments including CK(normal soil),T0(damaged soil),T1(damaged soil applied compound microbial fertilizer 1 with 0.7 mL/kg applied in dry soil),T2(damaged soil applied compound microbial fertilizer 2 with 0.7 mL/kg applied in dry soil),T3(damaged soil applied compound microbial fertilizer 3 with 1.1 g/kg applied in dry soil).The results showed that the activities of superoxide dismutase(SOD),aseorbateperoxidase(APX)and catalase(CAT)in tobacco plants of T0 treatment were 27.74%,30.43%and 39.67%which were lower than that of normal tobacco plants(CK),but their MDA content increased by 213.98%.However,different amounts of compound microbial fertilizer(T1,T2,T3)were applied to the soil affected tobacco plants.Compared with T0,SOD activity of tobacco plants in T1,T2 and T3 treatments increased by 16.60%,18.16%and 30.61%,and APX activity increased by 11.48%,16.40%and 33.35%after 20 days,respectively.And CAT activity increased by 25.23%,27.93%and 40.54%,while MDA content decreased by 8.22%,30.82%and 59.25%,respectively.The changes of three enzymes activity were consistent with that of MDA content in root system.The order of treatment effect was CK>T3>T2>T1>T0.Therefore,it was able to alleviate phytotoxicity of quchlorc to tobacco plants by adding suitable amounts of compound microbial fertilizer.