扶正益胃颗粒对顺铂处理荷瘤小鼠食欲及Ghrelin的影响

Effect of Fuzheng Yiwei Granule on Appetite and Ghrelin in Cisplatin-Treated Tumor-Bearing Mice

目的:通过构建荷瘤小鼠模型,探讨扶正益胃颗粒对顺铂处理荷瘤小鼠食欲及Ghrelin的影响。方法:40只C57BL/6小鼠皮下注射Lewis肺腺癌肿瘤细胞建立荷瘤模型,随机分为荷瘤组、顺铂组、扶正益胃颗粒组、醋酸甲地孕酮组,荷瘤组生理盐水每隔1天0.2 mL腹腔注射,其余各组小鼠每隔1天分别予以2.5 mg/mL顺铂腹腔注射。在此基础上荷瘤组和顺铂组予以生理盐水0.2 mL灌胃,扶正益胃颗粒组小鼠予以扶正益胃颗粒0.6 g/mL灌胃,醋酸甲地孕酮组小鼠予以4 mg/mL药物灌胃。连续给药14 d,采血后脱颈处死小鼠,取胃组织制成胃匀浆备用。观察测量小鼠饮食量,ELISA法检测血清中TNF-α、IL-6、IL-1及胃中Ghrelin含量水平。结果:给药后,扶正益胃颗粒组、醋酸甲地孕酮组小鼠食量较荷瘤组及顺铂组食量好(P<0.05)。相较于荷瘤组,顺铂组、扶正益胃颗粒组及醋酸甲地孕酮组TNF-α、IL-6、IL-1均明显降低(P<0.01)。与顺铂组比较,扶正益胃颗粒组TNF-α、IL-1降低明显(P<0.05)。与荷瘤组、顺铂组及醋酸甲地孕酮组比较,扶正益胃颗粒组Ghrelin含量明显升高(P<0.05)。结论:扶正益胃颗粒可提高顺铂处理荷瘤小鼠食欲,其机制可能与提高Ghrelin含量水平及降低TNF-α、IL-6、IL-1等炎症因子有关。

Objective:To investigate the effects of fuzheng yiwei granule on appetite and ghrelin in cisplatin-treated tumor-bear-ing mice by constructing a tumor-bearing mouse model.Methods:Forty C57BL/6 mice were subcutaneously injected with Lewis lung adenocarcinoma tumor cells to establish a tumor-bearing model,and were randomly divided into tumor-bearing group,cisplatin group,fuzheng yiwei granule group,and megestrol acetate group.The tumor-bearing group was intraperitone-ally injected with 0.2mL normal saline every other day,and the other groups were intraperitoneally injected with 2.5mg/mL cisplatin.On this basis,tumor-bearing group and cisplatin group were given normal saline 0.2mL by gavage,fuzheng yiwei granule group were given fuzheng yiwei granule 0.6g/mL,megestrol acetate group were given 4mg/mL.After continuous ad-ministration for 14days,the mice were sacrificed by removing the neck after blood collection,and gastric tissue was taken to make gastric homogenate.The levels of TNF-α,IL-6,IL-1in serum and ghrelin in stomach were detected by ELISA.Results:After administration,the food intake of mice in fuzheng yiwei granule group and megestrol acetate group was better than that in tumor-bearing group and cisplatin group(P<0.05).Compared with the tumor-bearing group,TNF-α,IL-6and IL-1were significantly decreased in Cisplatin group,fuzheng yiwei granule group and megestrol acetate group(P<0.01).Compared with cisplatin group,TNF-αand IL-1were significantly decreased in fuzheng yiwei granule group(P<0.05).Compared with the tumor-bearing group,cisplatin group and megestrol acetate group,the content of ghrelin in fuzheng yiwei granule group was significantly increased(P<0.05).Conclusion:Fuzheng yiwei granule can improve the appetite of tumor-bearing mice treated with cisplatin,and the mechanism may be related to the increase of ghrelin content and the decrease of TNF-α,IL-6,IL-1and other inflammatory factors.