生物信息学鉴定分析茄子脂肪酸去饱和酶(FAD)基因家族

Identification and Bioinformatics Analysis of Fatty Acid Desaturase(FAD)Gene Family in Eggplant(Solanum melongena L.)

脂肪酸去饱和酶(FAD)广泛分布于生物体内,其主要功能是在脂肪酸的生物合成过程中,从碳链中去除氢,从而产生碳碳双键。利用拟南芥FAD家族基因和茄子基因组数据,对茄子基因中可能含有的FAD基因进行鉴定和生物信息学分析。共鉴定出茄子FAD基因家族成员38个,其中FAB亚家族成员5个。对家族成员进行蛋白理化性质分析、系统进化分析和保守结构域分析,发现各亚家族成员间具有较为相似的保守基因序列和蛋白特性;基因结构分析表明,FAD家族成员间存在着不同的基因结构,但聚为一类的亚家族基因具有进化的保守性;染色体定位显示茄子FAD家族成员主要分布在5号染色体、6号染色体和4号染色体上。亚细胞定位预测茄子FAD家族成员主要分布在内质网、叶绿体和质膜上,FAB亚家族则全部定位在叶绿体上。

Fatty acid desaturase(FAD)is widely distributed in living organisms.Its main function is to remove hydrogen from the carbon chain during the biosynthesis of fatty acids,thus producing carbon-carbon double bonds.Using the data of the FAD family in Arabidopsis thaliana and the eggplant genome,the FAD genes in eggplant were identified and the bioinformatics of FAD genes were analyzed.Thirty-eight members of FAD gene family were identified,including five members of FAB subfamily.The analysis of protein physical and chemical properties,phylogenetic analysis and conserved domain analysis showed that there were relatively similar conserved gene sequences and protein characteristics among the members of each subfamily.There were different genetic structures among members of the FAD family,but the subfamily genes clustered together were evolutionarily conserved.Chromosome mapping showed that members of the FAD family were mainly distributed in chromosome 5,chromosome 6 and chromosome 4.The subcellular localization predicted that the members of the FAD family were mainly distributed in the endoplasmic reticulum,chloroplast and plasma membrane,while the FAB subfamilies were all located in the chloroplast.