‘金正日花’(Begonia×tuberhybrida Voss 'Kimjongilhwa')的愈伤组织诱导与芽分化

Callus Induction and Adventitious Bud Differentiation of Begoniaxtuberhybrida Voss‘Kimjongilhwa'

为提高‘金正日花’组织培养繁殖效率,试验以嫩叶作为外植体,探究了不同外植体消毒时间、采样时期、取材部位及培养基组分对其愈伤组织诱导和芽分化的影响。结果表明:‘金正日花’嫩叶用作外植体时,升汞消毒4.0 min和4.5 min均可,成活率分别达到61.11%和52.78%;适宜的嫩叶采样时期为10月;‘金正日花’嫩叶基部用作外植体培养更有利于其愈伤组织诱导和不定芽发生,愈伤组织诱导率为78.33%,不定芽发生率达83.01%;MS为‘金正日花’嫩叶用作外植体愈伤组织诱导与芽分化适宜的基本培养基;愈伤组织诱导与芽分化适宜的植物生长调节剂浓度组合为6-BA 1.5 mg/L+NAA 0.2 mg/L;6-BA 0.5 mg/L+NAA0.1 mg/L组合有利于不定芽的生长。

In order to improve the propagation efficiency of the tissue culture of‘Kimjongilia',the experiment used tender leaf as explant,and explored the effects of different disinfection time,sampling period,material selection and medium components on its callus induction and adventitious bud differentiation.The results showed when the tender leaf of‘Kimjongilia'was used as explant,it could be sterilized by mercury for 4.0 min and 4.5 min,and the survival rates were 61.11%and 52.78% respectively.The suitable sampling period for tender leaf was October.The base of the tender leaf of‘Kimjongilia'to be used as explant was more conducive to its callus induction and adventitious bud formation.The callus induction rate was 78.33%,and the adventitious bud incidence rated up to 83.01%.MS was a suitable basic medium for callus induction and adventitious bud differentiation of‘Kimjongilia'.The appropriate plant growth regulator concentration combination for callus induction and adventitious bud differentiation was 6-BA 1.5 mg/L+NAA 0.2 mg/L.The combination of 6-BA 0.5 mg/L+NAA 0.1 mg/L was beneficial to the growth of adventitious buds.