miR-153-3p靶向KLF7基因调控IL-6/STAT3通路抑制食管癌细胞的增殖和转移的机制研究

Mechanism of miR-153-3p Targeting KLF7 Gene Regulating IL-6/STAT3 Pathway Inhibiting Proliferation and Metastasis of Esophageal Cancer Cells

目的探讨miR-153-3p对食管癌细胞增殖和转移的影响及其作用机制。方法实时荧光定量PCR(RT-qPCR)检测miR-153-3p和Kruppel样因子7(KLF7)mRNA表达水平;蛋白质印迹(Western Blot)法检测KLF7、磷酸化信号转导与转录激活因子3(p-STAT3)、信号转导与转录激活因子3(STAT3)的表达;四甲基偶氮唑盐比色法(MTT)检测细胞活性;Transwell检测细胞迁移和侵袭;ELISA检测白细胞介素(IL-6)的表达;双荧光素酶报告基因检测KLF7和miR-153-3p的靶向。结果与正常食管上皮细胞HEEC相比,食管癌细胞EC9706、Eca-109、EC-1、TE-1中KLF7表达水平升高,miR-153-3p表达水平下降。过表达miR-153-3p和沉默KLF7可降低细胞活性和迁移、侵袭数量,降低p-STAT3表达水平,抑制IL-6的分泌。miR-153-3p靶向KLF7,上调KLF7能部分恢复miR-153-3p对EC9706细胞增殖、迁移、侵袭的作用。结论上调miR-153-3p可以靶向KLF7参与调节IL-6/STAT3通路抑制食管癌细胞增殖和转移。

Objective To investigate the effect of miR-153-3p on proliferation and metastasis of esophageal cancer cells and its mechanism.Methods Real-time quantitative PCR(RT-qPCR)was used to detect the expression of miR-153-3p and Kruppel-like factor 7(KLF7)mRNA;Western blot was used to detect KLF7,phosphorylation signal transducers and activators of transcription 3(p-STAT3),signal transducers and activators of transcription 3(STAT3)expression;tetramethylazozolium salt colorimetric assay(MTT)was used to detect cell viability;Transwell was used to detect cell migration and invasion;ELISA was used to detect expression of leukocyte(IL-6);dual luciferase reporter gene detects the targeting relationship of miR-153-3p and KLF7.Results Compared with normal esophageal epithelial cells HEEC,the expression of KLF7 in esophageal cancer cells EC9706,Eca-109,EC-1 and TE-1 were significantly increased,and the expression of miR-153-3p was significantly decreased.Overexpression miR-153-3p and silencing of KLF7 reduced cell viability and migration,invasion,decreased p-STAT3 expression,and inhibited IL-6 secretion.miR-153-3p targets KLF7,and overexpression of KLF7 can partially restore the inhibitory effect of miR-153-3p on EC9706 cell proliferation,migration and invasion.Conclusion Up-regulation of miR-153-3p can inhibit the proliferation and metastasis of esophageal cancer cells by targeting KLF7 and IL-6/STAT3 pathways.