miR-29a、PTEN、p53在育龄子宫内膜癌组织中的表达及与其术后复发转移的关系分析

Expressions of MiR-29a,PTEN and P53 in Cancer Tissues and Their Relationship with Postoperative Recurrence and Metastasis in Childbearing Age Patients with Endometrial Cancer

目的探讨育龄子宫内膜癌(EC)患者癌组织中微小RNA-29a(miR-29a)、张力蛋白同源物(PTEN)、肿瘤抑制基因(p53)表达与术后复发转移的关系。方法回顾性分析因EC进行手术治疗42例病理组织标本作为观察组,选取21例正常子宫内膜组织作为对照组。根据术后3年复发转移情况将观察组分为复发转移组19例和未复发转移组23例。荧光定量PCR检测EC癌组织和正常子宫内膜组织中miR-29a相对表达量;免疫组织化学技术检测EC癌组织和正常子宫内膜组织中PTEN、p53阳性表达率。结果观察组miR-29a相对表达量、PTEN阳性表达率低于对照组,p53阳性表达率高于对照组(P<0.05);育龄EC患者术后复发转移与肿瘤直径、浸润深度、远处转移、淋巴结转移和淋巴脉管转移有关(P<0.05);复发转移组miR-29a相对表达量、PTEN阳性表达率低于未复发转移组,p53阳性表达率高于未复发转移组(P<0.05);远处转移、miR-29a<1.92、PTEN阳性和P53阳性均为影响育龄EC患者术后复发转移的独立危险因素(P<0.05)。结论miR-29a、PTEN、p53在育龄EC患者癌组织中呈现表达异常,其异常表达是患者术后复发转移的独立危险因素。

Objective To explore the relationship between microRNA-29a(miR-29a),phosphat and tensin homolog deleted on chromosome ten(PTEN)and tumor suppressor gene(p53)in cancer tissues and postoperative recurrence,metastasis in childbearing age patients with endometrial cancer(EC).Methods A retrospective analysis was performed on the pathological tissue specimens(observation group)from 42 patients undergoing surgical treatment due to EC and normal endometrial tissues(control group)from 21 controls.According to presence or absence of recurrence and metastasis within 3 years after surgery,patients in observation group were divided into recurrence-metastasis group(19 cases)and non-recurrence-metastasis group(23 cases).The relative expression level of miR-29a in EC tissues and normal endometrial tissues was detected by fluorescence quantitative PCR.The positive expression rates of PTEN and p53 in EC tissues and normal endometrial tissues were detected by immunohistochemistry.Results The relative expression level of miR-29a and positive expression rate of PTEN in the observation group were lower than those in the control group,while positive expression rate of p53 was higher than that in control group(P<0.05).The postoperative recurrence and metastasis were related to tumor diameter,invasion depth,distant metastasis,lymph node metastasis and lymphovascular metastasis(P<0.05).The relative expression level of miR-29a and positive expression rate of PTEN in recurrence-metastasis group were lower than those in non-recurrence-metastasis group,while positive expression rate of p53 was higher than that in non-recurrence-metastasis group(P<0.05).Distant metastasis,miR-29a<1.92,PTEN positive and P53 positive were all independent risk factors of postoperative recurrence and metastasis in childbearing age patients with EC(P<0.05).Conclusion miR-29a,PTEN and p53 are abnormally expressed in cancer tissues in childbearing age patients with EC.And their abnormal expressions are independent risk factor of postoperative recurrence and metastasis.