阿拉伯木聚糖联合美多芭对帕金森病大鼠炎症因子TNF-α、IL-1β及IFN-γ表达的影响

Effects of araboxylan combined with madopar on the expression of inflammatory factors TNF-α,IL-1βand IFN-γin rats with Parkinson’s disease

目的探究阿拉伯木聚糖(araboxylan,AX)联合美多芭对鱼藤酮诱导的帕金森病(parkinson’s disease,PD)大鼠模型炎症因子TNF-α、IL-1β及IFN-γ的影响。方法42只大鼠随机选取28只进行PD造模,造模后分为帕金森模型组(PD组)、美多芭组(M组)和阿拉伯木聚糖组(AX组)、美多芭+阿拉伯木聚糖组(MX组)4个组;其余14只随机均分为空白组和溶剂组,每组7只。M组美多芭干预,AX组AX干预,MX组AX联合美多芭干预,灌胃14 d,每天1次。进行悬挂试验及爬杆实验,蛋白免疫印迹(Western Blotting,WB)检测黑质纹状体IFN-γ蛋白和免疫组化检测黑质纹状体IL-1β蛋白,采用酶联免疫吸附法(ELISA)检测各组血清TNF-α、IL-1β的表达。结果悬挂实验:AX、M组、MX组较PD组悬挂时间延长,四肢肌力改善,且MX组症状恢复更明显(P<0.01)。WB:AX组、M组、MX组与PD组比较,黑质纹状体IFN-γ的含量减少(P<0.05)。免疫组化:AX组、M组、MX组与PD组比较,黑质纹状体IL-1β表达量明显减少。ELISA:AX组、M组、MX组与PD组比较血清TNF-α降低。差异有统计学意义(P<0.05),MX组较AX组明显降低,差异有统计学意义(P<0.05)。结论阿拉伯木聚糖联合美多芭抑制鱼藤酮诱导的帕金森病大鼠炎症因子TNF-α、IL-1β及IFN-γ的释放,较好地改善了大鼠的神经功能。

Objective To investigate the effects of araboxylan(AX)combined with madopar on inflammatory factors TNF-α,IL-1βand IFN-γin rats with rotenone-induced Parkinson’s disease(PD).Methods Twenty-eight rats were randomly selected from forty-two rats for PD modeling,and they were divided into 4 groups after PD modeling:Parkinson’s model group(PD group),madopar group(M group),araboxylan group(AX group),and madopar+araboxylan group(MX group).The remaining 14 rats were randomly divided in to blank group and solvent group,with 7 rats in each group.The M group was treated with madopar;The AX group was treated with araboxylan,and the MX group was treated with a combination of madopar and araboxylan.All the rats were treated by intragastric gavage for 14 days,once a day.The suspension test and climbing-pole test were performed,and Western Blotting(WB)was used to detect IFN-γprotein in the substantia nigra striatum,while immunohistochemistry was used to detect IL-1βprotein in the substantia nigra striatum.Enzyme-linked immunosorbent assay(ELISA)was used to detect the expression of TNF-αand IL-1βin the serum of each group.Results In the suspension test,compared with those of the PD group,the hanging time was prolonged and limb muscle strength was improved in the AX,M and MX groups,and recovery of symptoms was more obvious in MX group(P<0.05);the results of WB showed that,compared with that of PD group,the concentration of IFN-γin the substantia nigra striatum decreased in the AX,M and MX groups(P<0.05).The results of Immunohistochemistry showed that,compared with that of the PD group,the expression of IL-1βin substantia nigra striatum in the AX group,M group,MX group significantly decreased.The results of ELISA showed that,compared with that of the PD group,the serum TNF-αdecreased in the AX,M and MX groups,the differences were statistically significant(P<0.05),MX group was significantly lower than AX group,the differences were statistically significant(P<0.05).Conclusion Araboxylan combined with madopar inhibit the release of inflammatory factors TNF-α,IL-1βand IFN-γin rats with rotenone-induced Parkinson’s disease,and improve the neurological function of rats quite well.