SPTBN2基因对膀胱癌5637细胞增殖、迁移和侵袭的影响

Effect of SPTBN2 gene on the proliferation,migration,and invasion of bladder cancer cell 5637

目的研究非红细胞血影蛋白β2(spectrin beta,non-erythrocytic 2,SPTBN2)对膀胱癌5637细胞增殖、迁移和侵袭能力的影响。方法通过体外实验研究SPTBN2对膀胱癌5637细胞生物学行为的影响。实验组(siRNA#1组、siRNA#2组)转染靶向SPTBN2 siRNA,阴性对照组转染用阴性siRNA,空白对照组中加入等量转染试剂。实时荧光定量PCR检测细胞中SPTBN2基因的表达水平,Western Blot检测细胞内SPTBN2蛋白的表达量。CCK-8法检测细胞增殖情况。划痕实验、Transwell侵袭实验检测细胞迁移、侵袭能力。结果转染SPTBN2 siRNA后,实验组的SPTBN2转录水平和蛋白表达量均低于阴性对照组和空白对照组。实验组的增殖、迁移和侵袭能力较阴性对照组降低,差异具有统计学意义(P<0.05)。结论利用靶向SPTBN2 siRNA成功构建低表达SPTBN2的5637细胞株,下调SPTBN2的表达可抑制膀胱癌5637细胞增殖、迁移和侵袭。

Objective To investigate the effects of spectrin beta non-erythrocytic 2(SPTBN2)on the proliferation,migration and invasion of bladder carcinoma cells 5637.Methods The effects of SPTBN2 on the biological behavior of bladder cancer cells 5637 were studied in vitro experiment.The experimental group(siRNA#1 group and siRNA#2 group)were transfected with targeted SPTBN2 siRNA,while the negative control group was transfected with negative siRNA,and the blank control group was added with the same amount of transfection reagents.Real-time quantitative PCR was employed to detect the expression of SPTBN2 gene in cells,and Western Blot was employed to detect the expression of SPTBN2 protein in cells.Cell proliferation was detected by CCK-8 assay.Cell migration and invasion ability were detected by wound healing assay and Transwell assay.Results After transfection of SPTBN2 siRNA,the transcription level and protein expression of SPTBN2 in experimental group were lower than those in the negative control group and the blank control group.The proliferation,migration and invasion abilities of cells in the experimental group decreased compared with the negative control group,and the differences were statistically significant(P<0.05).Conclusion Cell line 5637 with low expression of SPTBN2 is successfully constructed by targeted SPTBN2 siRNA,and the proliferation,migration and invasion of bladder cancer cell line 5637 can be inhibited by down-regulating SPTBN2 expression.