慢性胃炎患者肠道菌群分析

Analysis of intestinal microbiota in patients with chronic gastritis

目的探索慢性胃炎(CG)患者肠道菌群变化特点。方法采集我院青年CG患者(CG组)和健康人群(NC组)的粪便样品,对其细菌16S rDNA V3—V4区域进行扩增并进行高通量测序,然后运用多种生物信息学手段进行分析。结果CG组与NC组对象肠道菌群在门和科水平上均有不同之处,其中CG组对象有较高丰度的Actinobacteriota和较低丰度的Ruminococcaceae。CG组对象肠道菌群多样性及均一度均显著低于NC组(均P<0.05),但两者具有相似的丰富度水平。多元方差分析和相似性百分比分析均发现CG组和NC组对象肠道菌群有较大差异。Bifidobacterium、Blautia、Collinsella、Ruminococcus_torques_group和Streptococcus与CG患者密切相关。结论CG患者的肠道菌群存在较大变化,其中Bifidobacterium、Blautia等细菌与CG的发生相关。

Objective To investigate the alterations in intestinal microbiota in patients with chronic gastritis(CG).Methods The fecal samples were collected from CG cohort(CG group)and healthy negative control cohort(NC group).The 16S rDNA V3-V4 region of DNA was amplified before the high-throughput Illumina sequencing being conducted.Multiple bioinformatic analyses were performed for the subsequent investigation.Results The CG and NC groups were different at the levels of phyla and families,i.e.,more abundant Actinobacteriota and less abundant Ruminococcaceae were found in CG group.Significantly lower intestinal microbial diversity and evenness were determined in CG group than NC group,while the microbial richness was similar between the two groups.Both PERMANOVA and SIMPER analyses determined the differences between CG and NC cohorts.Multiple intestinal bacteria could contribute to the difference in the intestinal microbiota between CG and NC cohorts,among which Bifidobacterium,Blautia,Collinsella,Ruminococcus_torques_group and Streptococcus were associated with CG.Conclusion Great alterations in intestinal microbiota were determined in CG cohort,among which Bifidobacterium and Blautia etc.were associated with CG.