1株伪狂犬病病毒的基因变异及其对小鼠的致病性分析

Genetic Variation and Pathogenicity of a Pseudorabies Virus Variant Strain in Mice

本研究旨在了解我国猪伪狂犬病病毒(pseudorabies virus,PRV)流行毒株的分子遗传变异及毒力特点。对1株分离自1个接种了Bartha-K61疫苗的规模猪场的PRV ZJ株进行了主要糖蛋白基因序列(gB、gC和gE)的测定分析及对6周龄BALB/c小鼠的致病性研究。遗传变异分析显示,该毒株与我国2012年后的流行毒株高度同源,具有变异毒株基因特征,但gE基因的510位氨基酸出现了不同于变异株的独特变化S^(510) G。ZJ株对小鼠具有较高的致病性,攻毒小鼠呈现典型的神经症状,以100 LD_(50)感染小鼠,第5天死亡率达100%。本研究为进一步探索该病毒的毒株变异特点与致病机理奠定基础。

This study aimed to understand the molecular genetic variation and virulence characteristics of porcine pseudorabies virus(PRV)in China.The main glycoprotein gene sequences(gB,gC and gE)of PRV ZJ strain isolated from a pig farm inoculated with Bartha-K61 vaccine were determined.and its pathogenicity to 6-week-old mice was studied.Phylogenetic analysis results showed that this strain was highly homologous to epidemic strains in China after 2012,and had the genetic characteristics of the mutant strain.However,there was a unique change of S^(510) G in the amino acid position 510 of gE gene,which was different from the mutant strain.ZJ strain was highly pathogenic to BALB/c mice,and the challenged mice showed typical neurological symptoms.The mortality rate reached 100%on day 5 after the mice were infected with 100 LD_(50).This study laid a foundation for further exploration of the variation characteristics and pathogenesis of this virus.