有氧运动训练可通过调节脑血管生成及神经炎症反应改善大鼠缺血性脑损伤

Aerobic exercise repairs rats cerebral ischemic injury via regulating angiogenesis and neural inflammation

目的:探讨有氧运动改善大鼠缺血性脑损伤的可能作用机制。方法:将大鼠分为假手术(sham)组、脑中动脉栓塞局灶缺血性脑损伤(MCAO)组及有氧运动治疗(MCAO+E)组,每组12只;通过TTC染色及神经功能评分分别检测三组大鼠脑梗死体积及神经功能障碍情况,转棒实验评估三组大鼠平衡及运动协调能力;HE染色及TUNEL染色检测三组大鼠脑梗死灶细胞凋亡情况;运用FITC标记葡聚糖检测三组大鼠脑梗死灶血管密度,免疫荧光染色及ELISA法检测三组大鼠梗死灶炎症发生情况。结果:三组大鼠TTC染色表明,MCAO+E组梗死灶体积及神经功能评分较MCAO组均显著降低(P<0.01),转棒实验MCAO+E组大鼠滚筒持续时间较MCAO组显著延长(P<0.01);MCAO+E组梗死灶细胞凋亡较MCAO组显著减少(P<0.001),脑血管生成显著增多(P<0.001);MCAO组炎症细胞活化增多,功能增强,MCAO+E组可显著减少MCAO组引起的炎症细胞活化及功能增强(P<0.01)。结论:有氧运动训练可能通过促进血管生成抑制炎症反应,从而降低梗死灶细胞凋亡水平,最终改善大鼠缺血性脑损伤。

Objective:To investigate possible mechanisms of aerobic exercise in alleviating rats cerebral ischemia injury.Methods:Rats were divided into sham group,middle cerebral artery occlusion,focal ischemic brain injury(MCAO)group and aero‐bic exercise therapy(MCAO+E)group,with 12 rats in each group.Cerebral infarction volume and neurological dysfunction of rats in three groups were measured by TTC staining and neurological function score.Rotating rod test was used to evaluate balance and motor coordination ability of rats in three groups.HE staining and TUNEL staining were used to detect apoptosis of cerebral infarction of rats in three groups.FITC-dextran was used to detect vascular density of cerebral infarction of rats in three groups.Immunofluorescence staining and ELISA were used to detect occurrence of inflammation in infarcted area of rats in three groups.Results:TTC staining of rats in three groups showed that the infarct volume and neurological function score in MCAO+E group were significantly lower than those in MCAO group(P<0.01).Poller duration of rats in MCAO+E group was significantly longer than that in MCAO group(P<0.01).Com‐pared with MCAO group,apoptosis of infarct cells in MCAO+E group was significantly decreased(P<0.001),while cerebral angiogene‐sis was significantly increased(P<0.001);activation and function of inflammatory cells induced by MCAO group were increased,and MCAO+E group could significantly reduce the activation and function enhancement of inflammatory cells in MCAO group(P<0.01).Conclusion:Aerobic exercise training may improve the ischemic brain injury of rats by promoting angiogenesis and inhibiting inflamma‐tory reaction,thereby reducing the level of apoptosis in the infarct and finally improve the ischemic injury in rats.