miR-551a拮抗蟾毒灵对肝癌细胞增殖和转移的效应

miR-551a antagonizes effect of bufalin on proliferation and metastasis of liver cancer cells

目的:研究蟾毒灵对肝癌细胞增殖和转移影响的分子机制。方法:人肝癌细胞SK-Hep1分为NC组、蟾毒灵低、高剂量组、anti-miR-NC组、anti-miR-551a组、miR-NC组、miR-551a组、高剂量组+miR-NC组、高剂量组+miR-551a组。四甲基偶氮唑盐比色法(MTT)检测细胞增殖活性;流式细胞仪检测细胞凋亡;Transwell检测细胞迁移和侵袭;实时荧光定量PCR(RT-qPCR)检测miR-551a表达水平。结果:蟾毒灵处理的SK-Hep1细胞活性降低,细胞凋亡率升高,细胞迁移、侵袭数量减少,miR-551a表达水平降低(P<0.05)。抑制miR-551a表达后,SK-Hep1细胞活性降低,细胞凋亡率升高,细胞迁移、侵袭数量减少(P<0.05)。过表达miR-551a逆转了蟾毒灵对肝癌细胞的影响。结论:蟾毒灵可能通过抑制miR-551a的表达抑制肝癌细胞的增殖和转移,促进细胞凋亡。

Objective:To investigate the molecular mechanism of bufalin affecting the proliferation and metastasis of liver can‐cer cells.Methods:Human liver cancer cell SK-Hep1 were divided into NC group,bufalin low and high dose group,anti-miR-NC group,anti-miR-551a group,miR-NC group,miR-551a group,high-dose group+miR-NC group,high-dose group+miR-551a group.MTT was used to detect cell proliferation activity;flow cytometry to detect cell apoptosis;Transwell was used to detect cell migration and invasion;RT-qPCR was used to detect the expression level of miR-551a.Results:Bufalin-treated SK-Hep1 cell activity was de‐creased,the rate of apoptosis was increased,the number of cell migration and invasion were decreased,and the expression level of miR-551a was decreased(P<0.05).After inhibiting the expression of miR-551a,SK-Hep1 cell activity was decreased,cell apoptosis rate was increased,and the number of cell migration and invasion were decreased(P<0.05).Overexpression of miR-551a reversed the effect of bufalin on liver cancer cells.Conclusion:Bufalin may inhibit the proliferation and metastasis of liver cancer cells,and pro‐mote cell apoptosis by inhibiting the expression of miR-551a.