响应面法优化柴胡药渣中柴胡皂苷提取工艺

Optimization of Saikosaponin Extraction from Bupleuri Radix Based on Response Surface Methodology

为确立微波提取柴胡药渣中柴胡皂苷的最佳工艺,采用响应面实验设计优化了提取过程中的工艺参数.基于单因素实验对工艺参数进行初步筛选,进一步的采用响应面实验建立二阶回归模型,优化了提取时间、提取温度、料液比和甲醇体积百分比4个工艺参数.结果发现:提取温度和提取时间的交互作用、提取温度和甲醇体积百分比的交互作用、提取温度和料液比的交互作用对皂苷提取率的影响较为显著;优化最佳工艺参数提取温度为68℃、料液比为1∶59 g/mL、提取时间为30 min、甲醇体积百分比为60%.在该工艺参数下柴胡皂苷提取率最大,达到5.14 mg/g.

In order to get an optimal technology for saikosaponin extraction from Bupleuri Radix,the factors affecting the saikosaponin extraction rate were screened primitively by single factor experiment.The important parameters in the extraction process of saikosaponin,such as extraction time,microwave extraction temperature,solid-liquid ratio and methanol concentration,were optimized based on response surface design.The results show that the interactions of extraction temperature with time,with methanol concentration,and with solid-liquid ratio have evidently influence on the saikosaponin extraction rate.The optimum technological parameters verified by experiments present,when extraction time is 30 min,microwave extraction temperature is 68℃,solid-liquid ratio is 1∶59 g/mL and methanol concentration is 60%,the extraction rate of saponins is the most,can reach 5.14 mg/g with better repeatability.The achievement has laid a foundation for the industrialized extraction of saikosaponin.