乙酰基二肽-1鲸蜡酯的皮肤抗衰老作用及其机制

Anti-senescence effect of acetyl dipeptide-1 cetyl ester on skin and its mechanism

乙酰基二肽-1鲸蜡酯(acetyl dipeptide-1 cetyl ester,AD-1)是乙酸和十六烷基醇与精氨酸和酪氨酸组成的人工合成肽,具有一定的抗炎和提高皮肤屏障功能的作用,已被用于敏感肌化妆品中。该成分虽然也被用于抗衰老化妆品中,但缺乏公开的系统科学研究。本研究通过评价AD-1的体外抗氧化和抗糖基化作用、建立UVA反复照射真皮原代成纤维细胞的光老化模型,探讨其在光老化细胞中的抗衰老活性。采用MTT法测定AD-1对细胞活力的影响;RT-qPCR法检测AD-1和UVA对衰老相关p21、p53、MMPs、IL6、Col1、Col3以及自噬相关p62、ATG5、ATG7 mRNA表达的影响;Western blot法检测AD-1和UVA对衰老及自噬相关p16、p21、p53、Col1、LC3B、p62蛋白水平的影响;β-半乳糖苷酶染色和MDC染色评价细胞衰老损伤,并通过荧光探针DCFH-DA检测细胞内活性氧水平。实验结果显示,AD-1干预可以减少UVA诱导的细胞损伤和mRNA表达异常,改善p16、p21、p53、Col1、LC3B、p62蛋白水平异常。结果表明,AD-1具有一定的体外抗氧化、抗糖基化能力,同时可能通过激活自噬保护UVA诱导的光老化细胞,具有较好的抗衰老活性。

Acetyl dipeptide-1 cetyl ester(AD-1)is a synthetic peptide composed of acetic acid and cetyl alcohol with arginine and tyrosine,which has certain anti-inflammatory and skin barrier enhancement effects,has been used in cosmetics for sensitive skin.Meanwhile,the ingredient has also been used in anti-aging cosmetics,but there is a lack of published scientific evidence on anti-senescence aspect.In this study,we investigated the related effects of AD-1 by evaluating its in vitro antioxidant and antiglycation efficacies.Furthermore,we established a photoaging model on primary rat dermal fibroblasts by repeated exposures to UVA irradiation.MTT assay was used to detect the effects of AD-1 on the cell viability.RT-qPCR was used to determine the effects of AD-1 on the mRNA levels of senescence-related p21,p53,MMPs,IL6,Col1,Col3 and autophagy-related p62,ATG5,ATG7.Western blot was used to detect the effects of AD-1 on the protein levels of p16,p21,p53,Col1,LC3B and p62.SA-β-gal was performed to indicate senescence level of the cell.MDC was performed to indicate autophagy level.Intracellular reactive oxygen species were monitored by fluorescent probes DCFH-DA.The results showed that AD-1 could reduce UVA-induced the cell damage and regulate the abnormal expression of mRNA levels.It allesuggested that AD-1 has not only antioxidant and antiglycation effects but also can activate autophagy to achieve anti-senescence effect.