SNP-9对鱼藤酮所致帕金森病细胞模型的作用及机制研究

Effects and mechanisms of SNP-9 on Parkinson's disease cell model induced by rotenone

本文研究SNP-9对帕金森病(Parkinson’s disease,PD)细胞模型的作用和机制。使用鱼藤酮损伤SH-SY5Y细胞建立PD细胞模型;通过MTT法检测鱼藤酮和SNP-9对细胞活力的影响;Hoechst/PI双染法检测鱼藤酮和SNP-9对细胞凋亡的影响;DCFH-DA探针检测鱼藤酮和SNP-9对细胞活性氧(reactive oxygen species,ROS)水平的影响;Western blot检测鱼藤酮和SNP-9对酪氨酸羟化酶(tyrosine hydroxylase,TH)、α-突触核蛋白(α-synuclein,α-syn)、Bcl-2、Bax蛋白水平的影响。研究结果显示,SNP-9能够缓解鱼藤酮诱导的SH-SY5Y细胞活力、TH和α-syn水平、细胞凋亡、ROS水平,以及细胞凋亡相关蛋白Bax/Bcl-2的异常。研究结果提示SNP-9可能通过调控凋亡相关通路,进而缓解鱼藤酮导致的神经细胞损伤。

In this article,the effects and mechanisms of SNP-9 on Parkinson's disease(PD)cell model were investigated.SH-SY5Y cells were treated with rotenone to establish PD cell model;the effects of rotenone and SNP-9 on cell viability were detected by MTT assay;Hoechst/PI double staining assay was used to detect the effects of rotenone and SNP-9 on cell apoptosis;DCFH-DA probe was used to detect the effects of rotenone and SNP-9 on cellular reactive oxygen species(ROS)levels;and Western blot was used to detect the effects of rotenone and SNP-9 on protein levels of tyrosine hydroxylase(TH),α-synuclein(α-syn),Bcl-2 and Bax.The results showed that SNP-9 could alleviate abnormalities in cell viability,levels of TH andα-syn,apoptosis,ROS and apoptotic relative protein Bax/Bcl-2 induced by rotenone.Our findings suggest that SNP-9 may alleviate rotenoneinduced injury in neuronal cells by regulating cell apoptosis related pathway.