表面活性剂对采油菌生长的影响

The Influence of Surfactant Stress on the Growth of Oil-Producing Bacteria

为了研究表面活性剂驱油后储层微生物的适应性,本研究选择油田上广泛使用的表面活性剂:脂肪醇聚氧乙烯醚硫酸钠(AES)、十二烷基硫酸钠(SDS)和聚乙二醇辛基苯基醚(Triton X-100),确定采油菌在不同浓度的表面活性剂胁迫条件下的Gompertz模型,并通过拟合参数来衡量拟合度。结果表明,当AES和SDS的浓度高于4×10^(3)mg/L时,生长迟缓期明显延长,最大生长量降低。阴沟肠杆菌对Triton X-100表现出较高的耐受性,AES和SDS浓度低于4×10^(3)mg/L时,拟合效果良好。本研究阐明了微生物提高采收率(MEOR)中表面活性剂对采油菌生长和代谢的作用机制,为微生物驱油的研究提供了理论依据。

In order to study the adaptation of reservoir microorganisms after surfactant drive,the surfactants widely used on the oilfield:sodium aliphatic polyoxyethylene ether sulfate(AES),sodium dodecyl sulfate(SDS),and polyethylene glycol octylphenyl ether(Triton X-100)were selected in this study,the Gompertz model for oil recovery bacteria under different concentrations of surfactant stress conditions was determined and the fitting parameters to measure the goodness of fit.The results showed that the growth retardation period was significantly prolonged and the maximum growth was reduced when the concentrations of AES and SDS were higher than 4×10^(3) mg/L.Enterobacter cloacae showed high tolerance to Triton X-100 and good fit at AES and SDS concentrations below 4×10^(3) mg/L.This study elucidates the mechanism of surfactant action on the growth and metabolism of oil recovery bacteria in microbial enhanced oil recovery(MEOR),and provides a theoretical basis for the study of microbial oil drive.