改性壳聚糖/海藻酸止血海绵的制备及其快速凝血性能研究

Fabrication of modified chitosan/alginate hemostatic sponge and its performance for quick hemostasis

通过一步冷冻干燥法将多孔海藻酸钙海绵基材与烷基化壳聚糖结合制备了改性多孔止血海绵。该改性海绵具有疏松多孔结构,可促进血液快速浸润其多孔结构。而海绵中的改性壳聚糖可通过其烷基侧链与血液中的血红细胞等作用促进血液快速凝固。结果表明,相比未改性的海绵基材,改性止血海绵展现出快速凝血能力,其体外凝血指数在10 s内低至7.22%;同时,该改性止血海绵亦展现出良好的血液相容性,其溶血率仅为1.4%。

A kind of modified porous hemostatic sponge is prepared by integrating porous Ca-alginate sponges with alkyl-modified chitosan via one-step lyophilization process.The sponge has highly porous structure which enables blood to quickly penetrate into their porous structure upon contacting with the blood.Meanwhile,alkyl-modified chitosan polymers in the porous structure allow interaction between red blood cells in the blood and their alkyl side chains to promote the blood to clot.The results indicate that compared with the unmodified sponge,the modified porous hemostatic sponge can exhibit a rapid hemostatic property,and its blood clotting index reaches as low as 7.22%within 10 seconds.Meanwhile,this modified porous hemostatic sponge shows a good hemocompatibility,with a low hemolysis ratio of 1.4%.