负载酶/CaCO_(3)颗粒的聚合物凝胶球的制备及其催化性能研究

Preparation of polymer gel spheres loaded with enzyme/CaCO_(3)particles and study on their catalytic performance

通过微通道悬滴乳化法构建内含酶/CaCO_(3)的单体液滴并将其作为模板制备负载酶/CaCO_(3)颗粒的聚合物凝胶球。该凝胶球的水凝胶高分子网络结构及其内部的介孔结构CaCO_(3)颗粒具有良好的传质性能,可为CaCO_(3)颗粒中所封装的酶分子提供有效保护,在赋予其良好催化活性的同时增强其稳定性和环境耐受性。以醇脱氢酶为典型酶分子在凝胶球中进行封装,并利用醇脱氢酶将甲醛催化转化为甲酸的反应评估该凝胶球的酶固定化性能。结果表明,由于具有良好的固定保护和传质性能,该凝胶球可展现出与游离酶相当的酶催化活性;分别经80℃热水和紫外光照等苛刻条件处理后,该凝胶球仍能有效保持酶催化活性,展现出了良好的稳定性和环境耐受性。

Monomer droplets containing enzyme/CaCO_(3)are constructed through micro-channel suspension emulsification method,and used as templates to prepare polymer gel spheres loaded with enzyme/CaCO_(3)particles.The hydrogel polymer network structure of the spheres and the mesoporous structures of CaCO_(3)particles loaded in the spheres endow the spheres with excellent mass transfer,and can supply good protection to enzyme molecules immobilized in CaCO_(3)particles,thus providing the enzymes with excellent enzymatic catalytic property,enhanced stability and environmental tolerance.Through encapsuling alcohol dehydrogenase in the polymer gel spheres,the enzyme immobilization performance of the spheres is evaluated in catalytic conversion of formaldehyde to formic acid.It is verified from the results that these spheres can deliver similar catalytic activity as that of free alcohol dehydrogenase due to their good protection and mass transfer properties.After treated with hot water(80℃)and UV irradiation,these spheres still remains their catalytic activity effectively,showing good stability and environmental tolerance.