桔梗多糖对嗜黏蛋白阿克曼氏菌生长及代谢的影响

Effect of Platycodon Grandiflorum Polysaccharide on the Growth and Metabolism of Akkermansia Muciniphila

目的探究桔梗多糖对嗜黏蛋白阿克曼氏菌(Akk菌)生长和代谢的影响。方法将Akk菌与桔梗多糖进行体外共孵育,测定Akk菌的生长曲线;采用LC-MS/MS法测定Akk菌液中短链脂肪酸(SCFAs)的含量;采用非靶向代谢组学/脂质组学对Akk菌的代谢物进行检测和分析。结果适量的桔梗多糖可以促进Akk菌的生长,增加Akk菌液中SCFAs的含量,尤其是丁酸;此外,桔梗多糖还可以调节Akk菌液中9类脂质和18种其它代谢物。结论桔梗多糖可以促进Akk菌的富集并调节其代谢。为桔梗多糖作为Akk菌的益生元提供理论依据。

OBJECTIVE To investigate the effect of Platycodon grandiflorum polysaccharides on regulating the growth and metabolism of Akkermansia muciniphila(A.muciniphila).METHODS Platycodon grandiflorum polysaccharides were co-incubated with A.muciniphila in vitro and the growth curve was determined;Use LC-MS/MS to determine the content of short-chain fatty acids(SCFAs);Non-targeted metabolomics and non-targeted lipidomics were performed to study the changes of metabolic profiles in A.muciniphila after Platycodon grandiflorum polysaccharides treatment.RESULTS Platycodon grandiflorum polysaccharides could promote the growth of A.muciniphila,and increase the content of short-chain fatty acids in A.muciniphila,especially butyrate.Furthermore,Platycodon grandiflorum polysaccharides also remodeled the metabolic profiles of A.muciniphila,as 9 lipids and 18 other metabolites were regulated.CONCLUSION Platycodon grandiflorum polysaccharides can enrich A.muciniphila and regulate its metabolism.This study provides a theoretical basis for harnessing Platycodon grandiflorum polysaccharides as prebiotics of A.muciniphila.