干式荧光免疫分析仪检测胃泌素-17及胃蛋白酶原在幽门螺杆菌感染与慢性萎缩性胃炎诊断中的应用

Significance of Gastrin-17 and Pepsinogen Expression in the Serum of Patients with Helicobacter Pylori Infection and Chronic Atrophic Gastritis

目的:通过调查分析干式荧光免疫分析仪在检测幽门螺杆菌感染与慢性萎缩性胃炎患者血清中胃泌素-17及胃蛋白酶原的应用价值。方法:选择2020年6月~2021年5月中南大学湘雅二院桂林医院消化内科住院的100例慢性萎缩性胃炎患者作为此次实验研究的对象,分别采用干式荧光免疫分析仪(观察组)和尿素呼气试验检测(对照组)患者幽门螺杆菌感染情况,统计检测情况以及两种方式下检测结果的准确性、相关性等情况。结果:通过对患者进行病理学和诊断结果证明100例中,有41例患者感染幽门螺杆菌,感染率达41%,其中,尿素呼气试验检测(对照组)检出37例,干式荧光免疫分析仪检出40例。在干式荧光免疫分析仪检测下,感染幽门螺杆菌的慢性萎缩性胃炎患者胃泌素17和胃蛋白酶原水平明显上升,而未感染幽门螺旋杆菌的慢性萎缩性胃炎患者,胃泌素17和胃蛋白酶原水平下降。观察组干式荧光免疫分析仪检测结果和对照组尿素呼气试验检测结果一致。结论:干式荧光免疫分析仪具有携带方便、操作简单、结果准确、特别适用于批量样本及入户走访现场进行胃泌素-17及胃蛋白酶原检测,进而为临床上诊断幽门螺杆菌与慢性萎缩性胃炎提供依据。

Objective:This study investigated and analyzed the application value of dry-fluorescent immunoanalyzer in detecting gastrin-17 and pepsinogen in the serum of patients with Helicobacter pylori infection and chronic atrophic gastritis.Methods:Choose from June 2020 to May 2021,the second people’s hospital of 100 patients with chronic atrophic gastritis as the object of the experimental study,respectively with dry fluorescent immunoanalyzer(experimental group)and urea breath test(control)Helicobacter pylori infection,statistical detection and the accuracy of the test results,correlation,and so on and so forth.Results:Among the 100 cases confirmed by pathology and diagnosis,41 patients were infected with Helicobacter pylori,and the infection rate reached 41%,among which 37 cases were detected by urea breath test(control group)and 40 cases were detected by dry fluorescent immunoanalyzer.By addition,gastrin 17 and pepsinogen levels increased in patients with chronic atrophic gastritis infected with H.pylori,while gastrin 17 and pepsinogen levels decreased in patients with chronic gastritis not infected with H.pylori.The results of the experimental group were consistent with the urea breath test of the control group.Conclusion:Dry fluorescent immunoanalyzer is easy to carry,easy to operate,accurate results,especially suitable for batch samples and home visits for gastrin-17 and pepsinogen detection,and then provides a basis for clinical diagnosis of Helicobacter pylori and chronic atrophic gastritis.