氧化石墨烯对铅胁迫下菌丝体生长的影响

Effects of Graphene Oxide on Mycelial Growth Under Lead Stress

以大球盖菇、双孢蘑菇、毛头鬼伞、蛹虫草、梯棱羊肚菌和六妹羊肚菌为试材,采用平板培养的方法,研究50、100、200、350 mg·L^(-1)铅浓度胁迫对6种真菌生长的影响,并在铅胁迫下生长最佳的菌株中添加氧化石墨烯,观察对菌丝生长的影响,以期为后期利用氧化石墨烯和大型真菌联合修复环境中重金属污染提供参考依据。结果表明:在6种菌株中,六妹羊肚菌的平均生长速率最快,当培养基铅浓度在350 mg·L^(-1)时,六妹羊肚菌的平均生长速率可达23.93 mm·d^(-1),且在100 mg·L^(-1)铅浓度胁迫下,添加氧化石墨烯能促进菌丝体的生长,但随着铅浓度的升高,氧化石墨烯对铅毒害的屏蔽作用降低。

Taking Stropharia rugosoannulata,Agaricus bisporus,Coprinus comatus,Cordyceps militaris,Morchella importuna and Morchella importuna as test materials,the method of plate culture was used,the growth effects of six fungi under lead stress of 50,100,200,350 mg·L^(-1) were studied,and graphene oxide was added to the strains with the best growth under lead stress to observe the effects on the growth of mycelia,in order to provide reference for the later use of graphene oxide and large fungi to jointly repair the heavy metal pollution in the environment.The results showed that the average growth rate of Morchella sextus was the fastest among the six strains.When the lead concentration in the medium was 350 mg·L^(-1),the average growth rate of Morchella sextus was23.93 mm·d^(-1).Under the lead concentration stress of 100 mg·L^(-1),the addition of graphene oxide could promote the growth of mycelia,but with the increasing of lead concentration,the shielding effect of graphene oxide on lead toxicity decreased.