基于电场调控的胆固醇基液晶薄膜对成纤维细胞生长分化的影响

Effect of Electric Field-Regulating Cholesterol-based Liquid Crystal Films on the Growth and Differentiation of Fibroblasts

通过外加电场对胆固醇-g-聚乳酸低聚物(Cholesteryl-oligo(L-lactic acid),CLA)薄膜的液晶有序性进行调节,并评价其对成纤维细胞黏附、增殖和分化的影响。采用差示扫描量热仪检测CLA聚合物液晶性;以外加直流电场调控薄膜液晶有序性,X射线衍射仪和偏光显微镜检测电场调控下CLA薄膜液晶有序性的改变;Cell Counting Kit-8试剂盒检测成纤维细胞在CLA薄膜上的黏附与增殖行为,免疫荧光染色法和蛋白免疫印迹法检测成纤维细胞表型转化特征蛋白α-平滑肌肌动蛋白(α-Smooth muscle actin, α-SMA)表达水平。结果表明,CLA聚合物具有液晶性,外加电场可通过诱导CLA液晶分子的有序排列调控CLA薄膜液晶性,CLA薄膜液晶性增强有利于成纤维细胞的增殖与α-SMA蛋白表达。说明电场调控可在不改变CLA聚合物薄膜基质材料组成的前提下,通过增加薄膜的液晶有序性,促进成纤维细胞向肌成纤维细胞表型转化。

The liquid crystal ordering of cholesterol-g-polylactic acid oligomer(CLA)film is regulated by external direct-current electric field,and its effects on the adhesion,proliferation and differentiation of fibroblasts are evaluated.The liquid crystalline properties of CLA polymer aredetected by differential scanning calorimeter.The CLA films is prepared under a direct-current electric field with different intensities,and the liquid crystal ordering is detected by X-ray diffractometer and polarized light microscope.The adhesion and proliferation of fibroblasts on the CLA films are detected by a Cell Counting Kit-8 kit.The expression ofα-smooth muscle actin(α-SMA)is detected by immunofluorescence staining and western blotting.The results show that CLA polymer has liquid crystal property,and the external electric field could regulate the liquid crystal property of CLA films isbeneficial to the proliferation of fibroblasts and the expression of α-SMAprotein. These results suggest that electric field could promote the phenotypic transformation of fibroblaststo myofibroblasts by increasing the liquid crystal ordering of CLA polymer film, without changing thecomposition of matrix materials.