榛蘑蛋白提取工艺的优化研究

Optimization of Extraction Technology of Protein from Armillaria mellea

蛋白质是一种保证机体健康的营养物质,具有多种功效。以榛蘑粉末为原料,榛蘑蛋白提取率为指标,采用碱提和超声分步提取榛蘑蛋白,利用响应面法优化榛蘑蛋白提取工艺,等电点法和盐析法相结合沉淀榛蘑蛋白。结果表明,液料比47∶1、碱提pH 10.0、碱提时间1.55 h、碱提温度80℃、超声功率200 W、超声pH 9.0、超声温度30℃的条件下,榛蘑蛋白的提取率为(76.59±0.23)%。榛蘑蛋白质沉淀的方法为先进行等电点沉淀(pI=3.7),然后用90%的饱和硫酸铵进行盐析。试验表明碱提和超声分步提取的方法与传统碱提法相比,可有效提高蛋白提取率。等电点法结合盐析法可提高蛋白得率。该试验结果为榛蘑蛋白的开发利用奠定了基础。

Protein is a nutrient that keeps the body healthy and has various functions.Using Armillaria mellea powder as the raw material and Armillaria mellea protein extraction rate as the index,Armillaria mellea protein is extracted by alkaline extraction and ultrasonic stepwise extraction,Armillaria mellea protein extraction process is optimized by response surface method,and Armillaria mellea protein is precipitated by the combination of isoelectric point method and salting out method.The results show that under the conditions of liquid-solid ratio 47∶1,alkaline extraction pH 10.0,alkaline extraction time 1.55 h,alkaline extraction temperature 80℃,ultrasonic power 200 W,ultrasonic pH 9.0 and ultrasonic temperature 30℃,the extraction rate of Armillaria mellea protein is(76.59±0.23)%.The precipitation method of Armillaria mellea protein is isoelectric point precipitation(pI=3.7)firstly,and then salting out with 90%saturated ammonium sulfate.The results show that compared with the traditional alkaline extraction method,the method of alkaline extraction and ultrasonic stepwise extraction could effectively improve the extraction rate of protein.Isoelectric point method combined with salting out method can improve the protein yield.The results have laid a foundation for the development and utilization of protein from Armillaria mellea.