云南玉龙鼠类携带贝氏柯克斯体的基因分型研究

GENOTYPING OF COXIELLA BURNETII CIRCULATING IN MICE IN YULONG COUNTY,YUNNAN PROVINCE

为更好地了解云南省鼠类中贝氏柯克斯体Coxiella burnetii的流行情况,以便制定针对Q热的预防和控制措施,本研究采用巢式PCR方法检测云南省玉龙县捕获的鼠类携带贝氏柯克斯体的情况,并对阳性样本的贝氏柯克斯体进行多间隔区序列分型(multispacer sequence typing,MST)和多位点可变数目的串联重复分析(multilocus variable number tandem repeats analysis,MLVA)。结果显示,在玉龙共捕获到49只野生鼠类,其中24只携带贝氏柯克斯体(48.98%,24/49)。基因分型结果显示阳性样本携带贝氏柯克斯体的MST型别可分为MST16和一种新的MST基因型别,但未能获得最终的MLVA基因型别。本研究表明云南玉龙的鼠类携带新的贝氏柯克斯体MST基因型别,可为我国Q热的溯源提供一定的理论依据和数据支撑。

In order to investigate the distribution of Coxiella burnetii in mice and establish prevention and control measures for Q fever in Yunnan Province,C.burnetii carried in mice in Yulong County was detected using nested PCR.Then C.burnetii in positive sample was genotyped based on the multispacer sequence typing(MST)and the multilocus variable number tandem repeats analysis(MLVA).As a result,a total of 49 mice were collected from Yulong County,in which 24 mice(48.98%,24/49)were detected positive in C.burnetii-specific nested PCR.The positive samples of mice were then typed into 2 genotypes using MST,and 1 of the 2 genotypes was recognized as an unidentified one and the other was recognized as MST16.However,the allele sequences required for MLVA genotyping have not been obtained completely.Overall,a new MST type has been identified from wild mice collected from Yulong County,Yunnan Province,providing theoretical basis for the traceability of Q fever in China.