结膜松弛症动物模型的构建和评估

Construction and evaluation of animal models of conjunctivochalasis

目的:通过球结膜下注射基质金属蛋白酶(metalloproteinase,MMP)-1、3构建结膜松弛症的动物模型。方法:20只新西兰大白兔随机分为4组:对照组无干预措施;伪造模组球结膜下注射生理盐水;MMP-1组球结膜下注射MMP-1;MMP-3组球结膜下注射MMP-3。8周后观察眼前节照相和眼前节光学相干检查、泪液蕨类试验及结膜组织光镜检查结果。结果:对照组和伪造模组未见结膜皱褶形成,泪液羊齿状结晶样结构完整,光镜下未见胶原和弹力纤维破坏;MMP-1组可见结膜皱褶形成,泪液羊齿状结晶样结构破坏,光镜下见胶原和弹力纤维破坏;MMP-3组未见结膜皱褶形成,泪液羊齿状结晶样结构部分破坏,光镜下见胶原和弹力纤维部分破坏。结论:球结膜下注射MMP-1可构建结膜松弛症1~2级的动物模型。

Objective:To establish an animal model of conjunctivochalasis by subconjunctival injection of matrix metalloproteinase 1 and 3.Methods:New Zealand white rabbits were randomly divided into four groups,the control group had no intervention measures;Pseudo model group was injected susbconjunctival with normal saline;MMP-1 group was injected subconjunctival with matrix metalloproteinase 1;MMP-3 group was injected subconjunctival with matrix metalloproteinase 3.After 8 weeks,the results of anterior segment photography and optical coherence test,tear fern test and conjunctival tissue light microscopy were observed.Results:No conjunctival folds were observed in the control group and Pseudo model group,the lacrimal fern-shaped crystalline structures were intact,and no destruction of collagen and elastic fibers was found under the light microscope.In MMP-1 group,conjunctival folds were formed,lacrimal ferns crystalline structures were damaged,and collagen and elastic fibers were damaged under light microscope.No conjunctival folds were found in MMP-3 group,and the lacrimal ferns crystalline structure was partially destroyed,while the collagen and elastic fibers were partially destroyed under light microscope.Conclusion:Subconjunctival injection of matrix metalloproteinase-1 can construct the animal model of conjunctivochalasis of 1-2 grades.